PDBsum entry 5jgo

Transferase

PDB id: 5jgo

Contents

Protein chain

371 a.a.

Ligands

6JX

GOL ×2

EDO ×2

Metals

_ZN

Waters ×368

PDB id:

5jgo

Name:

Transferase

Title:

tRNA guanine transglycosylase (tgt) in co-crystallized complex with allyl (2-(methylamino)-8-oxo-7,8-dihydro-1h-imidazo[4,5-g]quinazolin- 6-yl)carbamate

Structure:

Queuine tRNA-ribosyltransferase. Chain: a. Synonym: guanine insertion enzyme,tRNA-guanine transglycosylase. Engineered: yes

Source:

Zymomonas mobilis subsp. Mobilis (strain atcc 31821 / zm4 / cp4). Organism_taxid: 264203. Strain: atcc 31821 / zm4 / cp4. Gene: tgt, zmo0363. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.37Å R-factor: 0.136 R-free: 0.162

Authors:

F.R.Ehrmann,A.Heine,G.Klebe

Key ref:

F.R.Ehrmann et al. Carbamate-Protected lin-Benzoguanines as promising candidates for improving solubility and membrane permeability. To be published, .

Date:

20-Apr-16 Release date: 10-May-17

Protein chain

P28720  (TGT_ZYMMO) -  Queuine tRNA-ribosyltransferase from Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)

Seq: 386 a.a.

Struc: 371 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.2.4.2.29 - tRNA-guanosine(34) preQ1 transglycosylase.
• Reaction: 7-aminomethyl-7-carbaguanine + guanosine₃₄ in tRNA = 7-aminomethyl-7- carbaguanosine₃₄ in tRNA + guanine

7-aminomethyl-7-carbaguanine (Bound ligand (Het Group name = 6JX) matches with 50.00% similarity) + guanosine(34) in tRNA = 7-aminomethyl-7- carbaguanosine(34) in tRNA + guanine

Molecule diagrams generated from .mol files obtained from the KEGG ftp site