PDBsum entry 5igc

Hydrolase

PDB id: 5igc

Contents

Protein chain

129 a.a.

Ligands

THP

Metals

_CA

Waters ×95

PDB id:

5igc

Name:

Hydrolase

Title:

Crystal structure of staphylococcal nuclease variant delta+phs v23e/v66h at cryogenic temperature

Structure:

Thermonuclease. Chain: a. Fragment: unp residues 83-231. Synonym: tnase, micrococcal nuclease, staphylococcal nuclease. Engineered: yes. Mutation: yes

Source:

Staphylococcus aureus. Organism_taxid: 1280. Gene: nuc. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

1.80Å R-factor: 0.191 R-free: 0.225

Authors:

A.C.Robinson,A.Theodoru,J.L.Schlessman,B.Garcia-Moreno E.

Key ref:

A.C.Robinson et al. Crystal structure of staphylococcal nuclease variant delta+phs v23e/v66h at cryogenic temperature. To be published, .

Date:

27-Feb-16 Release date: 09-Mar-16

Protein chain

P00644  (NUC_STAAU) -  Thermonuclease from Staphylococcus aureus

Seq: 231 a.a.

Struc: 129 a.a.*

* PDB and UniProt seqs differ at 7 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.1.31.1 - micrococcal nuclease.
• Reaction: Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products.