PDBsum entry 5id2

Oxidoreductase

PDB id: 5id2

Contents

Protein chains

154 a.a.

Ligands

GOL ×2

ACT

Waters ×230

PDB id:

5id2

Name:

Oxidoreductase

Title:

Asymmetry in the active site of mycobacterium tuberculosis ahpe upon exposure to mycothiol

Structure:

Putative peroxiredoxin rv2238c. Chain: a, d. Synonym: thioredoxin reductase. Engineered: yes. Putative peroxiredoxin rv2238c. Chain: b, c. Synonym: thioredoxin reductase. Engineered: yes

Source:

Mycobacterium tuberculosis h37rv. Organism_taxid: 83332. Strain: h37rv. Atcc: atcc 25618. Gene: rv2238c, mtahpe. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

2.43Å R-factor: 0.188 R-free: 0.205

Authors:

A.Kumar,A.M.Balakrishna,G.Gruber

Key ref:

A.Kumar et al. (2016). Redox chemistry of Mycobacterium tuberculosis alkylhydroperoxide reductase E (AhpE): Structural and mechanistic insight into a mycoredoxin-1 independent reductive pathway of AhpE via mycothiol. Free Radic Biol Med, 97, 588-601. PubMed id: 27417938 DOI: 10.1016/j.freeradbiomed.2016.07.007

Date:

23-Feb-16 Release date: 03-Aug-16

Protein chains

P9WIE3  (AHPE_MYCTU) -  Alkyl hydroperoxide reductase E from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

Seq: 153 a.a.

Struc: 154 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.1.11.1.29 - mycoredoxin-dependent peroxiredoxin.
• Reaction: [mycoredoxin]-L-dithiol + a hydroperoxide = [mycoredoxin]-L-disulfide + an alcohol + H2O

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

DOI no: 10.1016/j.freeradbiomed.2016.07.007

Free Radic Biol Med 97:588-601 (2016)

PubMed id: 27417938

Redox chemistry of Mycobacterium tuberculosis alkylhydroperoxide reductase E (AhpE): Structural and mechanistic insight into a mycoredoxin-1 independent reductive pathway of AhpE via mycothiol.

A.Kumar, A.M.Balakrishna, W.Nartey, M.S.Manimekalai, G.Grüber.

ABSTRACT

No abstract given.