PDBsum entry 5hrh

Transferase/DNA

PDB id: 5hrh

Contents

Protein chains

174 a.a.

DNA/RNA

Ligands

DGT ×2

Metals

_MN ×4

Waters ×2

PDB id:

5hrh

Name:

Transferase/DNA

Title:

The crystal structure of asfvpolx(h115f/r127a mutant): 1nt-gap(p) dna2:dgtp ternary complex

Structure:

DNA polymerase beta-like protein. Chain: a, b. Synonym: po174l, polx. Engineered: yes. Mutation: yes. DNA (5'- d( Cp Gp Tp Tp Cp Tp Ap Tp Gp Tp Gp Tp Ap Cp Tp Cp Ap C)-3'). Chain: c, f. Engineered: yes.

Source:

African swine fever virus. Asfv. Organism_taxid: 10497. Gene: o174l. Expressed in: escherichia coli. Expression_system_taxid: 469008. Synthetic: yes. Synthetic construct. Organism_taxid: 32630.

Resolution:

3.00Å R-factor: 0.234 R-free: 0.273

Authors:

Y.Q.Chen,J.Zhang,J.H.Gan

Key ref:

Y.Q.Chen et al. The crystal structure of se-Asfvpolx(l52/163m mutant) complex with 1nt-Gap DNA1. To be published, .

Date:

23-Jan-16 Release date: 18-Jan-17

Protein chains

P42494  (DPOLX_ASFB7) -  Repair DNA polymerase X from African swine fever virus (strain Badajoz 1971 Vero-adapted)

Seq: 174 a.a.

Struc: 174 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

DNA/RNA chains

C-G-T-T-C-T-A-T-G-T-G-T-A-C-T-C-A-C 18 bases

G-T-G-A-G-T-A-C-A 9 bases

A-T-A-G-A-A-C-G 8 bases

C-G-T-T-C-T-A-T-G-T-G-T-A-C-T-C-A-C 18 bases

G-T-G-A-G-T-A-C-A 9 bases

A-T-A-G-A-A-C-G 8 bases

Enzyme reactions

• Enzyme class: E.C.2.7.7.7 - DNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site