PDBsum entry 5hpn

De novo protein

PDB id: 5hpn

Contents

Protein chains

87 a.a.

81 a.a.

Ligands

SO4

Waters ×17

PDB id:

5hpn

Name:

De novo protein

Title:

A circularly permuted pdua forming an icosahedral cage

Structure:

Permuted pdua. Chain: a, b, c, d, e. Engineered: yes

Source:

Synthetic construct. Organism_taxid: 32630. Expressed in: synthetic construct. Expression_system_taxid: 32630

Resolution:

2.51Å R-factor: 0.216 R-free: 0.256

Authors:

D.J.Leibly,J.Jorda,T.O.Yeates

Key ref:

J.Jorda et al. (2016). Structure of a novel 13 nm dodecahedral nanocage assembled from a redesigned bacterial microcompartment shell protein. Chem Commun (camb), 52, 5041-5044. PubMed id: 26988700 DOI: 10.1039/c6cc00851h

Date:

20-Jan-16 Release date: 06-Apr-16

Protein chains

No UniProt id for this chain

Struc: 87 a.a.

Protein chain

No UniProt id for this chain

Struc: 81 a.a.

DOI no: 10.1039/c6cc00851h

Chem Commun (camb) 52:5041-5044 (2016)

PubMed id: 26988700

Structure of a novel 13 nm dodecahedral nanocage assembled from a redesigned bacterial microcompartment shell protein.

J.Jorda, D.J.Leibly, M.C.Thompson, T.O.Yeates.

ABSTRACT

We report the crystal structure of a novel 60-subunit dodecahedral cage that results from self-assembly of a re-engineered version of a natural protein (PduA) from the Pdu microcompartment shell. Biophysical data illustrate the dependence of assembly on solution conditions, opening up new applications in microcompartment studies and nanotechnology.