PDBsum entry 5gyn

Hydrolase

PDB id: 5gyn

Contents

Protein chain

295 a.a.

Metals

_MG

Waters ×197

PDB id:

5gyn

Name:

Hydrolase

Title:

Crystal structure of human pyridoxal 5'-phosphate phosphatase (chronophin) mutant - c221s

Structure:

Pyridoxal phosphate phosphatase. Chain: a. Synonym: plp phosphatase,chronophin. Engineered: yes. Mutation: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: pdxp, cin, plp, plpp. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

2.00Å R-factor: 0.199 R-free: 0.234

Authors:

H.J.Lee,H.J.Cho,B.S.Kang

Key ref:

H.J.Lee et al. Crystal structure of human pyridoxal 5'-Phosphate phosphatase (chronophin) mutant - C221s. To be published, .

Date:

23-Sep-16 Release date: 27-Sep-17

Protein chain

Q96GD0  (PLPP_HUMAN) -  Chronophin from Homo sapiens

Seq: 296 a.a.

Struc: 295 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class 1: E.C.3.1.3.16 - protein-serine/threonine phosphatase.
• Reaction:
  1. O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate
  2. O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate
• Enzyme class 2: E.C.3.1.3.74 - pyridoxal phosphatase.
• Reaction: pyridoxal 5'-phosphate + H2O = pyridoxal + phosphate
• Cofactor: Mg(2+)

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site