PDBsum entry 5gqh

Hydrolase/hydrolase inhibitor

PDB id: 5gqh

Contents

Protein chains

901 a.a.

135 a.a.

PDB id:

5gqh

Name:

Hydrolase/hydrolase inhibitor

Title:

Cryo-em structure of paecas3-acrf3 complex

Structure:

Crispr-associated nuclease/helicase cas3 subtype i-f/ypest. Chain: a. Synonym: type i-f crispr-associated nuclease/helicase cas3. Engineered: yes. Anti-crispr protein 3. Chain: b, c. Synonym: acrf3,uncharacterized protein. Engineered: yes

Source:

Pseudomonas aeruginosa ucbpp-pa14. Organism_taxid: 208963. Strain: ucbpp-pa14. Gene: cas3. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Pseudomonas phage jbd5. Organism_taxid: 1223261. Gene: jbd5_035.

Authors:

X.Zhang,J.Ma,Y.Wang,J.Wang

Key ref:

J.Wang et al. (2016). A CRISPR evolutionary arms race: structural insights into viral anti-CRISPR/Cas responses. Cell Res, 26, 1165-1168. PubMed id: 27585537 DOI: 10.1038/cr.2016.103

Date:

07-Aug-16 Release date: 21-Sep-16

Protein chain

Q02ML8  (CAS3_PSEAB) -  CRISPR-associated nuclease/helicase Cas3 subtype I-F/YPEST from Pseudomonas aeruginosa (strain UCBPP-PA14)

Seq: 1076 a.a.

Struc: 901 a.a.

Protein chains

L7P7R7  (L7P7R7_9CAUD) -  Phage protein from Pseudomonas phage JBD5

Seq: 139 a.a.

Struc: 135 a.a.

Enzyme reactions

• Enzyme class 2: Chain A: E.C.3.1.-.-
• Enzyme class 3: Chain A: E.C.3.6.4.- - ?????

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

DOI no: 10.1038/cr.2016.103

Cell Res 26:1165-1168 (2016)

PubMed id: 27585537

A CRISPR evolutionary arms race: structural insights into viral anti-CRISPR/Cas responses.

J.Wang, J.Ma, Z.Cheng, X.Meng, L.You, M.Wang, X.Zhang, Y.Wang.

ABSTRACT

No abstract given.