PDBsum entry 5g0m

Hydrolase

PDB id: 5g0m

Contents

Protein chain

457 a.a.

Ligands

PO4 ×2

J13

Waters ×244

PDB id:

5g0m

Name:

Hydrolase

Title:

Beta-glucuronidase with an activity-based probe (n-acyl cyclophellitol aziridine) bound

Structure:

Beta-glucuronidase. Chain: a. Engineered: yes

Source:

Acidobacterium capsulatum. Organism_taxid: 33075. Atcc: 51196. Expressed in: escherichia coli. Expression_system_taxid: 469008. Expression_system_variant: gold.

Resolution:

1.80Å R-factor: 0.184 R-free: 0.213

Authors:

Y.Jin,L.Wu,J.B.Jiang,H.S.Overkleeft,G.J.Davies

Key ref:

Y.Jin et al. The structure of a bacteria beta-Glucuronidase in gh7 family. To be published, .

Date:

21-Mar-16 Release date: 12-Apr-17

Protein chain

C1F2K5  (C1F2K5_ACIC5) -  Beta-glucuronidase C-terminal domain-containing protein from Acidobacterium capsulatum (strain ATCC 51196 / DSM 11244 / BCRC 80197 / JCM 7670 / NBRC 15755 / NCIMB 13165 / 161)

Seq: 475 a.a.

Struc: 457 a.a.

Enzyme reactions

• Enzyme class: E.C.3.2.1.31 - beta-glucuronidase.
• Reaction: a beta-D-glucuronoside + H2O = D-glucuronate + an alcohol

beta-D-glucuronoside + H2O = D-glucuronate + alcohol (Bound ligand (Het Group name = J13) matches with 40.74% similarity)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site