PDBsum entry 5frr

Cell cycle

PDB id

5frr

Loading ...

Contents

			Protein chains

					677 a.a.

PDB id:

5frr

Links

Name:

Cell cycle

Title:

Structure of the pds5-scc1 complex and implications for cohesin function

Structure:

Sister chromatid cohesion protein pds5. Chain: a, b. Synonym: precocious dissociation of sisters protein 5, pds5. Engineered: yes

Source:

Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

5.80Å

R-factor:

0.249

R-free:

0.311

Authors:

K.W.Muir,M.Kschonsak,Y.Li,J.Metz,C.H.Haering,D.Panne

Key ref:

K.W.Muir et al. (2016). Structure of the Pds5-Scc1 Complex and Implications for Cohesin Function. Cell Rep, 14, 2116-2126. PubMed id: 26923589 DOI: 10.1016/j.celrep.2016.01.078

Date:

22-Dec-15

Release date:

02-Mar-16

PROCHECK

	Headers

	References

Protein chains

Q04264 (PDS5_YEAST) - Sister chromatid cohesion protein PDS5 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: Struc:

Seq: Struc:

Seq: Struc:					1277 a.a. 677 a.a.

Key:

PfamA domain

Secondary structure



		Enzyme reactions

Enzyme class:

E.C.?

[IntEnz] [ExPASy] [KEGG] [BRENDA]

DOI no: 10.1016/j.celrep.2016.01.078	Cell Rep 14:2116-2126 (2016)
PubMed id: 26923589

Structure of the Pds5-Scc1 Complex and Implications for Cohesin Function.
K.W.Muir, M.Kschonsak, Y.Li, J.Metz, C.H.Haering, D.Panne.

ABSTRACT

Sister chromatid cohesion is a fundamental prerequisite to faithful genome segregation. Cohesion is precisely regulated by accessory factors that modulate the stability with which the cohesin complex embraces chromosomes. One of these factors, Pds5, engages cohesin through Scc1 and is both a facilitator of cohesion, and, conversely also mediates the release of cohesin from chromatin. We present here the crystal structure of a complex between budding yeast Pds5 and Scc1, thus elucidating the molecular basis of Pds5 function. Pds5 forms an elongated HEAT repeat that binds to Scc1 via a conserved surface patch. We demonstrate that the integrity of the Pds5-Scc1 interface is indispensable for the recruitment of Pds5 to cohesin, and that its abrogation results in loss of sister chromatid cohesion and cell viability.