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PDBsum entry 5elc
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PDB id:
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Toxin
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Title:
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Cholera toxin el tor b-pentamer in complex with lewis-y
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Structure:
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Cholera enterotoxin subunit b. Chain: a, b, c, d, e, f, g, h, i, j. Synonym: cholera enterotoxin b chain,cholera enterotoxin gamma chain, choleragenoid. Engineered: yes
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Source:
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Vibrio cholerae o1. Organism_taxid: 127906. Gene: ctxb, toxb, vc_1456. Expressed in: vibrio. Expression_system_taxid: 662.
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Resolution:
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1.50Å
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R-factor:
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0.223
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R-free:
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0.260
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Authors:
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J.E.Heggelund,D.Burschowsky,U.Krengel
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Key ref:
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J.E.Heggelund
et al.
(2016).
High-Resolution Crystal Structures Elucidate the Molecular Basis of Cholera Blood Group Dependence.
Plos Pathog,
12,
e1005567.
PubMed id:
DOI:
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Date:
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04-Nov-15
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Release date:
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30-Mar-16
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PROCHECK
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Headers
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References
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P01556
(CHTB_VIBCH) -
Cholera enterotoxin subunit B from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
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Seq:
Struc:
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124 a.a.
103 a.a.
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Key: |
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Secondary structure |
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CATH domain |
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DOI no:
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Plos Pathog
12:e1005567
(2016)
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PubMed id:
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High-Resolution Crystal Structures Elucidate the Molecular Basis of Cholera Blood Group Dependence.
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J.E.Heggelund,
D.Burschowsky,
V.A.Bjørnestad,
V.Hodnik,
G.Anderluh,
U.Krengel.
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ABSTRACT
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Cholera is the prime example of blood-group-dependent diseases, with individuals
of blood group O experiencing the most severe symptoms. The cholera toxin is the
main suspect to cause this relationship. We report the high-resolution crystal
structures (1.1-1.6 Å) of the native cholera toxin B-pentamer for both
classical and El Tor biotypes, in complexes with relevant blood group
determinants and a fragment of its primary receptor, the GM1 ganglioside. The
blood group A determinant binds in the opposite orientation compared to
previously published structures of the cholera toxin, whereas the blood group H
determinant, characteristic of blood group O, binds in both orientations.
H-determinants bind with higher affinity than A-determinants, as shown by
surface plasmon resonance. Together, these findings suggest why blood group O is
a risk factor for severe cholera.
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