PDBsum entry 5eay

DNA binding protein

PDB id: 5eay

Contents

Protein chains

115 a.a.

12 a.a.

12 a.a.

PDB id:

5eay

Name:

DNA binding protein

Title:

Crystal structure of a dna2 peptide in complex with rpa 70n

Structure:

Replication protein a 70 kda DNA-binding subunit. Chain: a, b, c, d. Fragment: unp residues 3-120. Synonym: rp-a p70,replication factor a protein 1,rf-a protein 1, single-stranded DNA-binding protein. Engineered: yes. DNA replication atp-dependent helicase/nuclease dna2. Chain: e, f, g, h. Fragment: unp residues 1-17.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: rpa1, repa1, rpa70. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Organism_taxid: 9606

Resolution:

1.55Å R-factor: 0.233 R-free: 0.267

Authors:

C.Zhou,S.Pourmal,N.P.Pavletich

Key ref:

C.Zhou et al. (2015). Dna2 nuclease-Helicase structure, Mechanism and regul by rpa.. Elife, 4, . PubMed id: 26491943

Date:

17-Oct-15 Release date: 18-Nov-15

Protein chains

P27694  (RFA1_HUMAN) -  Replication protein A 70 kDa DNA-binding subunit from Homo sapiens

Seq: 616 a.a.

Struc: 115 a.a.

Protein chain

P51530  (DNA2_HUMAN) -  DNA replication ATP-dependent helicase/nuclease DNA2 from Homo sapiens

Seq: 1060 a.a.

Struc: 12 a.a.

Protein chains

P51530  (DNA2_HUMAN) -  DNA replication ATP-dependent helicase/nuclease DNA2 from Homo sapiens

Seq: 1060 a.a.

Struc: 12 a.a.

Enzyme reactions

• Enzyme class 2: Chains E, F, G, H: E.C.3.1.-.-
• Enzyme class 3: Chains E, F, G, H: E.C.3.6.4.12 - Dna helicase.
• Reaction: ATP + H2O = ADP + phosphate + H⁺

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site