PDBsum entry 5eax

Hydrolase/DNA

PDB id: 5eax

Contents

Protein chains

1046 a.a.

DNA/RNA

Ligands

SF4 ×2

ADP ×2

PDB id:

5eax

Name:

Hydrolase/DNA

Title:

Crystal structure of dna2 in complex with an ssdna

Structure:

DNA replication atp-dependent helicase/nuclease dna2. Chain: a, b. Synonym: DNA replication atp-dependent helicase-like homolog. Engineered: yes. Mutation: yes. DNA (5'- d( Tp Tp Tp Tp Tp Tp Tp Tp Tp Tp Tp Tp Tp Tp Tp Tp T)-3'). Chain: e, h. Engineered: yes

Source:

Mus musculus. Mouse. Organism_taxid: 10090. Gene: dna2, dna2l, kiaa0083. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Synthetic: yes. Synthetic construct. Organism_taxid: 32630

Resolution:

3.05Å R-factor: 0.223 R-free: 0.256

Authors:

C.Zhou,S.Pourmal,N.P.Pavletich

Key ref:

C.Zhou et al. (2015). Dna2 nuclease-Helicase structure, Mechanism and regul by rpa.. Elife, 4, . PubMed id: 26491943

Date:

17-Oct-15 Release date: 18-Nov-15

Protein chains

Q6ZQJ5  (DNA2_MOUSE) -  DNA replication ATP-dependent helicase/nuclease DNA2 from Mus musculus

Seq: 1062 a.a.

Struc: 1046 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

DNA/RNA chains

T-T-T-T-T-T-T-T-T-T-T-T-T-T-T-T-T 17 bases

T-T-T-T-T-T-T-T-T-T-T-T-T-T-T-T-T 17 bases

Enzyme reactions

• Enzyme class 2: E.C.3.1.-.-
• Enzyme class 3: E.C.3.6.4.12 - Dna helicase.
• Reaction: ATP + H2O = ADP + phosphate + H⁺

ATP + H2O = ADP (Bound ligand (Het Group name = ADP) corresponds exactly) + phosphate + H(+)

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site