PDBsum entry 5e4z

Lyase

PDB id: 5e4z

Contents

Protein chain

395 a.a.

Ligands

1PE

PEG

PLP

Metals

_NA

Waters ×106

PDB id:

5e4z

Name:

Lyase

Title:

Crystal structure of methionine gamma-lyase from citrobacter freundii with c115a substitution

Structure:

Methionine gamma-lyase. Chain: a. Engineered: yes. Mutation: yes

Source:

Citrobacter freundii. Organism_taxid: 546. Gene: ab180_17145, ab183_16780, tn42_08855, to64_18625. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

2.27Å R-factor: 0.175 R-free: 0.235

Authors:

S.V.Revtovich,A.D.Nikulin,N.V.Anufrieva,E.A.Morozova,T.V.Demidkina

Key ref:

S.V.Revtovich et al. Crystal structure of c115a mutant l-Methionine gamma- From citrobacter freundii. To be published, .

Date:

07-Oct-15 Release date: 12-Oct-16

Protein chain

A0A0A5P8W7  (A0A0A5P8W7_CITFR) -  L-methionine gamma-lyase from Citrobacter freundii

Seq: 398 a.a.

Struc: 395 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.4.4.1.11 - methionine gamma-lyase.
• Reaction: L-methionine + H2O = methanethiol + 2-oxobutanoate + NH4⁺

L-methionine + H2O = methanethiol (Bound ligand (Het Group name = PEG) matches with 40.00% similarity) + 2-oxobutanoate + NH4(+)

• Cofactor: Pyridoxal 5'-phosphate

Pyridoxal 5'-phosphate (Bound ligand (Het Group name = PLP) matches with 93.75% similarity)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site