PDBsum entry 5deh

Hydrolase

PDB id: 5deh

Contents

Protein chain

129 a.a.

Ligands

THP

Metals

_CA

Waters ×108

PDB id:

5deh

Name:

Hydrolase

Title:

Crystal structure of staphylococcal nuclease variant delta+phs n100d at cryogenic temperature

Structure:

Thermonuclease. Chain: a. Fragment: unp residues 83-231. Synonym: tnase, micrococcal nuclease, staphylococcal nuclease. Engineered: yes. Mutation: yes

Source:

Staphylococcus aureus. Organism_taxid: 1280. Gene: nuc. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

1.70Å R-factor: 0.170 R-free: 0.200

Authors:

L.A.Skerritt,P.C.Bell-Upp,M.A.Siegler,J.L.Schlessman,B.Garcia-Moreno E.

Key ref:

L.A.Skerritt et al. Crystal structure of staphylococcal nuclease variant delta+phs n100d at cryogenic temperature. To be published, .

Date:

25-Aug-15 Release date: 02-Sep-15

Protein chain

P00644  (NUC_STAAU) -  Thermonuclease from Staphylococcus aureus

Seq: 231 a.a.

Struc: 129 a.a.*

* PDB and UniProt seqs differ at 6 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.1.31.1 - micrococcal nuclease.
• Reaction: Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products.