PDBsum entry 5dc9

Protein binding

PDB id: 5dc9

Contents

Protein chains

100 a.a.

94 a.a.

Ligands

GOL ×5

IMD ×2

Waters ×232

PDB id:

5dc9

Name:

Protein binding

Title:

Crystal structure of monobody as25/abl1 sh2 domain complex, crystal b

Structure:

Tyrosine-protein kinase abl1. Chain: a. Synonym: abelson murine leukemia viral oncogene homolog 1,abelson tyrosine-protein kinase 1,proto-oncogenE C-abl,p150. Engineered: yes. As25 monobody. Chain: b. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: abl1, abl, jtk7. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

1.56Å R-factor: 0.138 R-free: 0.171

Authors:

J.B.Wojcik,A.Koide,S.Koide

Key ref:

J.Wojcik et al. (2016). Allosteric Inhibition of Bcr-Abl Kinase by High Affinity Monobody Inhibitors Directed to the Src Homology 2 (SH2)-Kinase Interface. J Biol Chem, 291, 8836-8847. PubMed id: 26912659 DOI: 10.1074/jbc.M115.707901

Date:

23-Aug-15 Release date: 02-Mar-16

Protein chain

P00519  (ABL1_HUMAN) -  Tyrosine-protein kinase ABL1 from Homo sapiens

Seq: 1130 a.a.

Struc: 100 a.a.

Protein chain

P02751  (FINC_HUMAN) -  Fibronectin from Homo sapiens

Seq: 2477 a.a.

Struc: 94 a.a.*

* PDB and UniProt seqs differ at 20 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain A: E.C.2.7.10.2 - non-specific protein-tyrosine kinase.
• Reaction: L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

DOI no: 10.1074/jbc.M115.707901

J Biol Chem 291:8836-8847 (2016)

PubMed id: 26912659

Allosteric Inhibition of Bcr-Abl Kinase by High Affinity Monobody Inhibitors Directed to the Src Homology 2 (SH2)-Kinase Interface.

J.Wojcik, A.J.Lamontanara, G.Grabe, A.Koide, L.Akin, B.Gerig, O.Hantschel, S.Koide.

ABSTRACT

No abstract given.