PDBsum entry 5dc0

Protein binding

PDB id: 5dc0

Contents

Protein chains

91 a.a.

98 a.a.

Waters ×55

PDB id:

5dc0

Name:

Protein binding

Title:

Crystal structure of monobody gg3/abl1 sh2 domain complex

Structure:

Fibronectin. Chain: a. Synonym: fn,cold-insoluble globulin,cig. Engineered: yes. Tyrosine-protein kinase abl1. Chain: b. Synonym: abelson murine leukemia viral oncogene homolog 1,abelson tyrosine-protein kinase 1,proto-oncogenE C-abl,p150. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: fn1, fn. Expressed in: escherichia coli. Expression_system_taxid: 469008. Gene: abl1, abl, jtk7.

Resolution:

2.23Å R-factor: 0.194 R-free: 0.244

Authors:

J.B.Wojcik,G.Grabe,S.Koide

Key ref:

J.Wojcik et al. (2016). Allosteric Inhibition of Bcr-Abl Kinase by High Affinity Monobody Inhibitors Directed to the Src Homology 2 (SH2)-Kinase Interface. J Biol Chem, 291, 8836-8847. PubMed id: 26912659 DOI: 10.1074/jbc.M115.707901

Date:

22-Aug-15 Release date: 02-Mar-16

Protein chain

P02751  (FINC_HUMAN) -  Fibronectin from Homo sapiens

Seq: 2477 a.a.

Struc: 91 a.a.*

Protein chain

P00519  (ABL1_HUMAN) -  Tyrosine-protein kinase ABL1 from Homo sapiens

Seq: 1130 a.a.

Struc: 98 a.a.

* PDB and UniProt seqs differ at 16 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain B: E.C.2.7.10.2 - non-specific protein-tyrosine kinase.
• Reaction: L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

DOI no: 10.1074/jbc.M115.707901

J Biol Chem 291:8836-8847 (2016)

PubMed id: 26912659

Allosteric Inhibition of Bcr-Abl Kinase by High Affinity Monobody Inhibitors Directed to the Src Homology 2 (SH2)-Kinase Interface.

J.Wojcik, A.J.Lamontanara, G.Grabe, A.Koide, L.Akin, B.Gerig, O.Hantschel, S.Koide.

ABSTRACT

No abstract given.