PDBsum entry 5d8s

Oxidoreductase

PDB id: 5d8s

Contents

Protein chains

(+ 6 more) 156 a.a.

Ligands

HEM ×7

Metals

__K ×3

Waters ×361

PDB id:

5d8s

Name:

Oxidoreductase

Title:

2.55a resolution structure of bfrb (e85a) from pseudomonas aeruginosa

Structure:

Ferroxidase. Chain: a, b, c, d, e, f, g, h, i, j, k, l. Fragment: bfrb. Engineered: yes. Mutation: yes

Source:

Pseudomonas aeruginosa (strain atcc 15692 / pao1 / 1c / prs 101 / lmg 12228). Organism_taxid: 208964. Strain: atcc 15692 / pao1 / 1c / prs 101 / lmg 12228. Gene: bfrb, pa3531. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.55Å R-factor: 0.184 R-free: 0.261

Authors:

S.Lovell,K.P.Battaile,Y.Wang,H.Yao,M.Rivera

Key ref:

Y.Wang et al. (2015). Characterization of the Bacterioferritin/Bacterioferritin Associated Ferredoxin Protein-Protein Interaction in Solution and Determination of Binding Energy Hot Spots. Biochemistry, 54, 6162-6175. PubMed id: 26368531 DOI: 10.1021/acs.biochem.5b00937

Date:

17-Aug-15 Release date: 23-Sep-15

Protein chains

Q9HY79  (Q9HY79_PSEAE) -  Bacterioferritin from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

Seq: 158 a.a.

Struc: 156 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.1.16.3.1 - ferroxidase.
• Reaction: 4 Fe²⁺ + O2 + 4 H⁺ = 4 Fe³⁺ + 2 H2O
• Cofactor: Cu cation

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Key reference

DOI no: 10.1021/acs.biochem.5b00937

Biochemistry 54:6162-6175 (2015)

PubMed id: 26368531

Characterization of the Bacterioferritin/Bacterioferritin Associated Ferredoxin Protein-Protein Interaction in Solution and Determination of Binding Energy Hot Spots.

Y.Wang, H.Yao, Y.Cheng, S.Lovell, K.P.Battaile, C.R.Midaugh, M.Rivera.

ABSTRACT

No abstract given.