PDBsum entry 5d8h

Ribosomal protein

PDB id: 5d8h

Contents

Protein chains

207 a.a.

158 a.a.

19 a.a.

DNA/RNA

Ligands

TRS ×3

Metals

_MG ×11

_NA ×4

Waters ×65

PDB id:

5d8h

Name:

Ribosomal protein

Title:

Crystal structure of the base of the ribosomal p stalk from methanococcus jannaschii with antibiotic thiostrepton

Structure:

23s ribosomal RNA. Chain: a. Engineered: yes. 50s ribosomal protein l10. Chain: b. Fragment: unp residues 9-221. Synonym: acidic ribosomal protein p0 homolog,mjal10. Engineered: yes. Mutation: yes.

Source:

Methanocaldococcus jannaschii. Organism_taxid: 2190. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Gene: rpl10, rplp0, mj0509. Expression_system_variant: pubs520. Gene: rpl11, mj0373. Streptomyces azureus. Organism_taxid: 146537

Resolution:

2.80Å R-factor: 0.210 R-free: 0.245

Authors:

A.G.Gabdulkhakov,I.V.Mitroshin,M.B.Garber

Key ref:

A.G.Gabdulkhakov et al. Crystal structure of the base of the ribosomal p stal methanococcus jannaschii with antibiotic thiostrepton. To be published, .

Date:

17-Aug-15 Release date: 24-Aug-16

Protein chain

P54049  (RL10_METJA) -  Large ribosomal subunit protein uL10 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

Seq: 338 a.a.

Struc: 207 a.a.*

Protein chain

P54030  (RL11_METJA) -  Large ribosomal subunit protein uL11 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

Seq: 161 a.a.

Struc: 158 a.a.

Protein chain

	P0C8P8  (THCL_STRAJ) -  Thiostrepton from Streptomyces azureus
	* PDB and UniProt seqs differ at 1 residue position (black cross)

DNA/RNA chain

G-C-C-U-A-A-G-A-C-A-G-C-G-G-G-G-A-G-G-U-U-G-G-C-U-U-A-G-A-A-G-C-A-G-C-C-A-U-C- 74 bases