PDBsum entry 5cse

Sugar binding protein

PDB id: 5cse

Contents

Protein chains

121 a.a.

Ligands

SVP ×2

Metals

_CL ×2

Waters ×50

PDB id:

5cse

Name:

Sugar binding protein

Title:

Streptavidin-s112y-k121e complexed with palladium-containing biotin ligand

Structure:

Streptavidin. Chain: a, b. Fragment: unp residues 37-158. Engineered: yes. Mutation: yes

Source:

Streptomyces avidinii. Organism_taxid: 1895. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.79Å R-factor: 0.225 R-free: 0.256

Authors:

A.D.Finke,L.Vera,M.Marsh,A.Chatterjee,T.R.Ward

Key ref:

A.Chatterjee et al. (2016). An enantioselective artificial Suzukiase based on the biotin-streptavidin technology. Chem Sci, 7, 673-677. PubMed id: 29896353

Date:

23-Jul-15 Release date: 04-Nov-15

Protein chains

P22629  (SAV_STRAV) -  Streptavidin from Streptomyces avidinii

Seq: 183 a.a.

Struc: 121 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Chem Sci 7:673-677 (2016)

PubMed id: 29896353

An enantioselective artificial Suzukiase based on the biotin-streptavidin technology.

A.Chatterjee, H.Mallin, J.Klehr, J.Vallapurackal, A.D.Finke, L.Vera, M.Marsh, T.R.Ward.

ABSTRACT

Introduction of a biotinylated monophosphine palladium complex within streptavidin affords an enantioselective artificial Suzukiase. Site-directed mutagenesis allowed the optimization of the activity and the enantioselectivity of this artificial metalloenzyme. A variety of atropisomeric biaryls were produced in good yields and up to 90% ee. The hybrid catalyst described herein shows comparable TOF to the previous aqueous-asymmetric Suzuki catalysts, and excellent stability under the reaction conditions to realize higher TON through longer reaction time.