PDBsum entry 5cnr

RNA

PDB id: 5cnr

Contents

DNA/RNA

Metals

_CL

PDB id:

5cnr

Name:

RNA

Title:

Crystal structure-guided design of self-assembling RNA nano triangles

Structure:

RNA (5'-r( Gp Up Cp Up Ap Cp Cp Cp Ap Cp Cp Up Cp Gp Cp C)- 3'). Chain: a, e. Engineered: yes. RNA (5'-r( Ap Gp Ap Gp Gp Ap Cp Gp Gp Cp G)-3'). Chain: b, f. Engineered: yes

Source:

Synthetic: yes. Seneca valley virus. Organism_taxid: 390157. Organism_taxid: 390157

Resolution:

2.59Å R-factor: 0.198 R-free: 0.244

Authors:

M.Boerneke,S.Dibrov,T.Hermann

Key ref:

M.A.Boerneke et al. (2016). Crystal-Structure-Guided Design of Self-Assembling RNA Nanotriangles. Angew Chem Int Ed Engl, 55, 4097-4100. PubMed id: 26914842 DOI: 10.1002/anie.201600233

Date:

18-Jul-15 Release date: 09-Mar-16

DNA/RNA chains

G-U-C-U-A-C-C-C-A-C-C-U-C-G-C-C 16 bases

A-G-A-G-G-A-C-G-G-C-G 11 bases

G-U-C-U-A-C-C-C-A-C-C-U-C-G-C-C 16 bases

A-G-A-G-G-A-C-G-G-C-G 11 bases

DOI no: 10.1002/anie.201600233

Angew Chem Int Ed Engl 55:4097-4100 (2016)

PubMed id: 26914842

Crystal-Structure-Guided Design of Self-Assembling RNA Nanotriangles.

M.A.Boerneke, S.M.Dibrov, T.Hermann.

ABSTRACT

RNA nanotechnology uses RNA structural motifs to build nanosized architectures that assemble through selective base-pair interactions. Herein, we report the crystal-structure-guided design of highly stable RNA nanotriangles that self-assemble cooperatively from short oligonucleotides. The crystal structure of an 81 nucleotide nanotriangle determined at 2.6 Å resolution reveals the so-far smallest circularly closed nanoobject made entirely of double-stranded RNA. The assembly of the nanotriangle architecture involved RNA corner motifs that were derived from ligand-responsive RNA switches, which offer the opportunity to control self-assembly and dissociation.