PDBsum entry 5cbk

Signaling protein

PDB id: 5cbk

Contents

Protein chain

271 a.a.

Ligands

PE3 ×2

Metals

_CL

_MG

Waters ×304

PDB id:

5cbk

Name:

Signaling protein

Title:

Crystal structure of the strigolactone receptor shhtl5 from striga hermonthica

Structure:

Shhtl5. Chain: a. Engineered: yes

Source:

Striga hermonthica. Organism_taxid: 68872. Gene: shhtl5. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

2.46Å R-factor: 0.171 R-free: 0.226

Authors:

P.J.Stogios,O.Onopriyenko,V.Yim,A.Savchenko

Key ref:

S.Toh et al. (2015). Structure-function analysis identifies highly sensitive strigolactone receptors in Striga. Science, 350, 203-207. PubMed id: 26450211 DOI: 10.1126/science.aac9476

Date:

01-Jul-15 Release date: 21-Oct-15

Protein chain

A0A0M5I297  (A0A0M5I297_STRHE) -  Hyposensitive to light 5 from Striga hermonthica

Seq: 271 a.a.

Struc: 271 a.a.

DOI no: 10.1126/science.aac9476

Science 350:203-207 (2015)

PubMed id: 26450211

Structure-function analysis identifies highly sensitive strigolactone receptors in Striga.

S.Toh, D.Holbrook-Smith, P.J.Stogios, O.Onopriyenko, S.Lumba, Y.Tsuchiya, A.Savchenko, P.McCourt.

ABSTRACT

Strigolactones are naturally occurring signaling molecules that affect plant development, fungi-plant interactions, and parasitic plant infestations. We characterized the function of 11 strigolactone receptors from the parasitic plant Striga hermonthica using chemical and structural biology. We found a clade of polyspecific receptors, including one that is sensitive to picomolar concentrations of strigolactone. A crystal structure of a highly sensitive strigolactone receptor from Striga revealed a larger binding pocket than that of the Arabidopsis receptor, which could explain the increased range of strigolactone sensitivity. Thus, the sensitivity of Striga to strigolactones from host plants is driven by receptor sensitivity. By expressing strigolactone receptors in Arabidopsis, we developed a bioassay that can be used to identify chemicals and crops with altered strigolactone levels.