PDBsum entry 5b6c

Hydrolase/protein binding

PDB id: 5b6c

Contents

Protein chains

174 a.a.

11 a.a.

Waters ×274

PDB id:

5b6c

Name:

Hydrolase/protein binding

Title:

Structural details of ufd1 binding to p97

Structure:

Transitional endoplasmic reticulum atpase. Chain: a. Fragment: unp residues 21-191. Synonym: ter atpase,15s mg(2+)-atpase p97 subunit,valosin-containing protein,vcp. Engineered: yes. Peptide from ubiquitin fusion degradation protein 1 homolog. Chain: b.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: vcp. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: ufd1l.

Resolution:

1.55Å R-factor: 0.160 R-free: 0.200

Authors:

L.T.M.Le,J.K.Yang

Key ref:

L.T.Le et al. (2016). Structural Details of Ufd1 Binding to p97 and Their Functional Implications in ER-Associated Degradation. Plos One, 11, e0163394. PubMed id: 27684549 DOI: 10.1371/journal.pone.0163394

Date:

26-May-16 Release date: 04-Jan-17

Protein chain

P55072  (TERA_HUMAN) -  Transitional endoplasmic reticulum ATPase from Homo sapiens

Seq: 806 a.a.

Struc: 174 a.a.*

Protein chain

Q92890  (UFD1_HUMAN) -  Ubiquitin recognition factor in ER-associated degradation protein 1 from Homo sapiens

Seq: 307 a.a.

Struc: 11 a.a.

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class 1: Chain A: E.C.3.6.4.6 - vesicle-fusing ATPase.
• Reaction: ATP + H2O = ADP + phosphate + H⁺
• Enzyme class 2: Chain B: E.C.?

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1371/journal.pone.0163394

Plos One 11:e0163394 (2016)

PubMed id: 27684549

Structural Details of Ufd1 Binding to p97 and Their Functional Implications in ER-Associated Degradation.

L.T.Le, W.Kang, J.Y.Kim, O.T.Le, S.Y.Lee, J.K.Yang.

ABSTRACT

No abstract given.