PDBsum entry 5b58

Metal binding protein

PDB id: 5b58

Contents

Protein chains

331 a.a.

258 a.a.

265 a.a.

PDB id:

5b58

Name:

Metal binding protein

Title:

Inward-facing conformation of abc heme importer bhuuv in complex with periplasmic heme binding protein bhut from burkholderia cenocepacia

Structure:

Putative hemin abc transport system, membrane protein. Chain: a, b. Synonym: heme importer transmembrane subunit bhuu. Engineered: yes. Hemin import atp-binding protein hmuv. Chain: c, d. Synonym: heme importer atp-binding subunit bhuv. Engineered: yes. Putative hemin transport system, substrate-binding protein.

Source:

Burkholderia cenocepacia j2315. Organism_taxid: 216591. Strain: j2315. Gene: hmuu, bcam2629. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: hmuv, bcam2630. Gene: hmut, bcam2628.

Resolution:

3.21Å R-factor: 0.271 R-free: 0.310

Authors:

Y.Naoe,N.Nakamura,A.Doi,Y.Shiro,H.Sugimoto

Key ref:

Y.Naoe et al. (2016). Crystal structure of bacterial haem importer complex in the inward-facing conformation. Nat Commun, 7, 13411. PubMed id: 27830695 DOI: 10.1038/ncomms13411

Date:

25-Apr-16 Release date: 23-Nov-16

Protein chains

B4EKB4  (B4EKB4_BURCJ) -  Hemin ABC transport system, membrane protein from Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610)

Seq: 362 a.a.

Struc: 331 a.a.

Protein chains

B4EKB5  (B4EKB5_BURCJ) -  Hemin ABC transporter ATP-binding protein from Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610)

Seq: 273 a.a.

Struc: 258 a.a.

Protein chain

B4EKB3  (B4EKB3_BURCJ) -  Hemin transport system, substrate-binding protein from Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610)

Seq: 305 a.a.

Struc: 265 a.a.

Enzyme reactions

• Enzyme class 1: Chains A, B, T: E.C.?
• Enzyme class 2: Chains C, D: E.C.3.6.3.- - ?????

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

DOI no: 10.1038/ncomms13411

Nat Commun 7:13411 (2016)

PubMed id: 27830695

Crystal structure of bacterial haem importer complex in the inward-facing conformation.

Y.Naoe, N.Nakamura, A.Doi, M.Sawabe, H.Nakamura, Y.Shiro, H.Sugimoto.

ABSTRACT

No abstract given.