PDBsum entry 5b0c

Lyase

PDB id

5b0c

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Contents

			Protein chains

					100 a.a.

			Waters ×229

PDB id:

5b0c

Links

Name:

Lyase

Title:

Polyketide cyclase oac from cannabis sativa, y27f mutant

Structure:

Olivetolic acid cyclase. Chain: a, b. Engineered: yes. Mutation: yes

Source:

Cannabis sativa. Hemp. Organism_taxid: 3483. Gene: oac. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.60Å

R-factor:

0.195

R-free:

0.230

Authors:

X.Yang,T.Matsui,T.Mori,I.Abe,H.Morita

Key ref:

X.Yang et al. (2016). Structural basis for olivetolic acid formation by a polyketide cyclase from Cannabis sativa. Febs J, 283, 1088-1106. PubMed id: 26783002 DOI: 10.1111/febs.13654

Date:

28-Oct-15

Release date:

27-Jan-16

PROCHECK

	Headers

	References

Protein chains

I6WU39 (OLIAC_CANSA) - Olivetolic acid cyclase from Cannabis sativa

Seq: Struc:					101 a.a. 100 a.a.*

Key:

PfamA domain

Secondary structure

CATH domain

* PDB and UniProt seqs differ at 1 residue position (black cross)



		Enzyme reactions

Enzyme class:

E.C.4.4.1.26 - olivetolic acid cyclase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

3,5,7-trioxododecanoyl-CoA = olivetolate + CoA + H⁺

3,5,7-trioxododecanoyl-CoA	=	olivetolate	+	CoA	+	H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Key reference

DOI no: 10.1111/febs.13654	Febs J 283:1088-1106 (2016)
PubMed id: 26783002

Structural basis for olivetolic acid formation by a polyketide cyclase from Cannabis sativa.
X.Yang, T.Matsui, T.Kodama, T.Mori, X.Zhou, F.Taura, H.Noguchi, I.Abe, H.Morita.

ABSTRACT

No abstract given.