PDBsum entry 5amp

Hydrolase

PDB id: 5amp

Contents

Protein chain

438 a.a.

Ligands

NAG-NAG

NAG ×2

GOL ×2

PEG ×2

Waters ×467

PDB id:

5amp

Name:

Hydrolase

Title:

Geotrichum candidum cel7a apo structure at 2.1a

Structure:

Cellobiohydrolase i. Chain: a. Fragment: catalytic domain, residues 18-456. Synonym: cellobiohydrolase cel7a. Ec: 3.2.1.176

Source:

Galactomyces candidum. Organism_taxid: 1173061. Strain: 3c

Resolution:

2.12Å R-factor: 0.149 R-free: 0.194

Authors:

A.S.Borisova,J.Stahlberg

Key ref:

A.S.Borisova et al. (2015). Sequencing, biochemical characterization, crystal structure and molecular dynamics of cellobiohydrolase Cel7A from Geotrichum candidum 3C. Febs J, 282, 4515-4537. PubMed id: 26367132 DOI: 10.1111/febs.13509

Date:

11-Mar-15 Release date: 23-Sep-15

Protein chain

A0A088T0J9  (A0A088T0J9_GEOCN) -  Glucanase (Fragment) from Geotrichum candidum

Seq: 535 a.a.

Struc: 438 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class 2: E.C.3.2.1.- - ?????
• Enzyme class 3: E.C.3.2.1.91 - cellulose 1,4-beta-cellobiosidase (non-reducing end).
• Reaction: Hydrolysis of 1,4-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains.

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

DOI no: 10.1111/febs.13509

Febs J 282:4515-4537 (2015)

PubMed id: 26367132

Sequencing, biochemical characterization, crystal structure and molecular dynamics of cellobiohydrolase Cel7A from Geotrichum candidum 3C.

A.S.Borisova, E.V.Eneyskaya, K.S.Bobrov, S.Jana, A.Logachev, D.E.Polev, A.L.Lapidus, F.M.Ibatullin, U.Saleem, M.Sandgren, C.M.Payne, A.A.Kulminskaya, J.Ståhlberg.

ABSTRACT

No abstract given.