PDBsum entry 5a5t

Hydrolase

PDB id: 5a5t

Contents

Protein chains

600 a.a.

558 a.a.

420 a.a.

272 a.a.

324 a.a.

216 a.a.

373 a.a.

366 a.a.

PDB id:

5a5t

Name:

Hydrolase

Title:

Structure of mammalian eif3 in the context of the 43s preinitiation complex

Structure:

Eukaryotic translation initiation factor 3 subunit a. Chain: a. Synonym: eif3a, eukaryotic translation initiation factor 3 subunit 10, eif-3-theta. Eukaryotic translation initiation factor 3 subunit c. Chain: c. Synonym: eif3c, eukaryotic translation initiation factor 3 subunit 8, eif3 p110. Eukaryotic translation initiation factor 3 subunit e.

Source:

Oryctolagus cuniculus. Rabbit. Organism_taxid: 9986. Cell_line: reticulcytes. Tissue: blood. Tissue: blood

Authors:

A.Des-Georges,V.Dhote,L.Kuhn,C.U.T.Hellen,T.V.Pestova,J.Frank, Y.Hashem

Key ref:

A.des Georges et al. (2015). Structure of mammalian eIF3 in the context of the 43S preinitiation complex. Nature, 525, 491-495. PubMed id: 26344199 DOI: 10.1038/nature14891

Date:

21-Jun-15 Release date: 09-Sep-15

Protein chain

G1SMZ5  (G1SMZ5_RABIT) -  Eukaryotic translation initiation factor 3 subunit A from Oryctolagus cuniculus

Seq: 1362 a.a.

Struc: 600 a.a.

Protein chain

G1U971  (G1U971_RABIT) -  Eukaryotic translation initiation factor 3 subunit C from Oryctolagus cuniculus

Seq: 913 a.a.

Struc: 558 a.a.*

Protein chain

G1SUC8  (G1SUC8_RABIT) -  Eukaryotic translation initiation factor 3 subunit E from Oryctolagus cuniculus

Seq: 462 a.a.

Struc: 420 a.a.

Protein chain

No UniProt id for this chain

Struc: 272 a.a.

Protein chain

G1ST95  (G1ST95_RABIT) -  Eukaryotic translation initiation factor 3 subunit H from Oryctolagus cuniculus

Seq: 363 a.a.

Struc: 324 a.a.

Protein chain

G1T3L2  (G1T3L2_RABIT) -  Eukaryotic translation initiation factor 3 subunit K from Oryctolagus cuniculus

Seq: 218 a.a.

Struc: 216 a.a.

Protein chain

G1SED9  (G1SED9_RABIT) -  Eukaryotic translation initiation factor 3 subunit L from Oryctolagus cuniculus

Seq: 607 a.a.

Struc: 373 a.a.

Protein chain

G1SLW8  (G1SLW8_RABIT) -  Eukaryotic translation initiation factor 3 subunit M from Oryctolagus cuniculus

Seq: 374 a.a.

Struc: 366 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chain F: E.C.3.4.19.12 - ubiquitinyl hydrolase 1.
• Reaction: Thiol-dependent hydrolysis of ester, thiolester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

DOI no: 10.1038/nature14891

Nature 525:491-495 (2015)

PubMed id: 26344199

Structure of mammalian eIF3 in the context of the 43S preinitiation complex.

A.des Georges, V.Dhote, L.Kuhn, C.U.Hellen, T.V.Pestova, J.Frank, Y.Hashem.

ABSTRACT

During eukaryotic translation initiation, 43S complexes, comprising a 40S ribosomal subunit, initiator transfer RNA and initiation factors (eIF) 2, 3, 1 and 1A, attach to the 5'-terminal region of messenger RNA and scan along it to the initiation codon. Scanning on structured mRNAs also requires the DExH-box protein DHX29. Mammalian eIF3 contains 13 subunits and participates in nearly all steps of translation initiation. Eight subunits having PCI (proteasome, COP9 signalosome, eIF3) or MPN (Mpr1, Pad1, amino-terminal) domains constitute the structural core of eIF3, to which five peripheral subunits are flexibly linked. Here we present a cryo-electron microscopy structure of eIF3 in the context of the DHX29-bound 43S complex, showing the PCI/MPN core at ∼6 Å resolution. It reveals the organization of the individual subunits and their interactions with components of the 43S complex. We were able to build near-complete polyalanine-level models of the eIF3 PCI/MPN core and of two peripheral subunits. The implications for understanding mRNA ribosomal attachment and scanning are discussed.