PDBsum entry 5a5h

Translation

PDB id: 5a5h

Contents

Protein chains

168 a.a.

210 a.a.

195 a.a.

Waters ×409

PDB id:

5a5h

Name:

Translation

Title:

The crystal structure of the gst-like domains complex of eprs c92sc105sc123s mutant-aimp2

Structure:

Bifunctional glutamate/proline--tRNA ligase. Chain: a, c, e, g. Fragment: gst-like domain. Synonym: bifunctional aminoacyl-tRNA synthetase, cell proliferation- inducing gene 32 protein, glutamatyl-prolyl-tRNA synthetase, glutamyl-tRNA synthetase, glurs, prolyl-tRNA synthetase, glutamyl- prolyl-tRNA synthetase. Engineered: yes. Mutation: yes.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 37762. Expression_system_variant: b834(de3). Expression_system_taxid: 469008.

Resolution:

2.32Å R-factor: 0.174 R-free: 0.227

Authors:

H.Y.Cho,B.S.Kang

Key ref:

H.Y.Cho and b.s.kang The crystal structure of the gst-Like domains complex eprs c92sc105sc123s mutant-Aimp2. To be published, .

Date:

18-Jun-15 Release date: 29-Jun-16

Protein chains

P07814  (SYEP_HUMAN) -  Bifunctional glutamate/proline--tRNA ligase from Homo sapiens

Seq: 1512 a.a.

Struc: 168 a.a.*

Protein chains

Q13155  (AIMP2_HUMAN) -  Aminoacyl tRNA synthase complex-interacting multifunctional protein 2 from Homo sapiens

Seq: 320 a.a.

Struc: 210 a.a.

Protein chains

Q13155  (AIMP2_HUMAN) -  Aminoacyl tRNA synthase complex-interacting multifunctional protein 2 from Homo sapiens

Seq: 320 a.a.

Struc: 195 a.a.

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class 1: Chains A, C, E, G: E.C.6.1.1.15 - proline--tRNA ligase.
• Reaction: tRNA(Pro) + L-proline + ATP = L-prolyl-tRNA(Pro) + AMP + diphosphate
• Enzyme class 2: Chains A, C, E, G: E.C.6.1.1.17 - glutamate--tRNA ligase.
• Reaction: tRNA(Glu) + L-glutamate + ATP = L-glutamyl-tRNA(Glu) + AMP + diphosphate

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site