PDBsum entry 5u9p

Oxidoreductase

PDB id: 5u9p

Contents

Protein chains

258 a.a.

Ligands

NAP ×4

TLA ×8

EDO ×10

Waters ×1160

PDB id:

5u9p

Name:

Oxidoreductase

Title:

Crystal structure of a gluconate 5-dehydrogenase from burkholderia cenocepacia j2315 in complex with NADP and tartrate

Structure:

Gluconate 5-dehydrogenase. Chain: a, b, c, d. Engineered: yes

Source:

Burkholderia cenocepacia (strain atcc baa-245 / dsm 16553 / lmg 16656 / nctc 13227 / j2315 / cf5610). Organism_taxid: 216591. Strain: atcc baa-245 / dsm 16553 / lmg 16656 / nctc 13227 / j2315 / cf5610. Atcc: baa-245. Gene: idno, bcal3386. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

1.65Å R-factor: 0.136 R-free: 0.164

Authors:

Seattle Structural Genomics Center For Infectious Disease (Ssgcid)

Key ref:

J.Abendroth et al. Crystal structure of a gluconate 5-Dehydrogenase from burkholderia cenocepacia j2315 in complex with NADP a tartrate. To be published, .

Date:

17-Dec-16 Release date: 18-Jan-17

Protein chains

B4EEX4  (B4EEX4_BURCJ) -  Gluconate 5-dehydrogenase from Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610)

Seq: 257 a.a.

Struc: 258 a.a.

Enzyme reactions

• Enzyme class: E.C.1.1.1.69 - gluconate 5-dehydrogenase.
• Reaction:
  1. D-gluconate + NADP⁺ = 5-dehydro-D-gluconate + NADPH + H⁺
  2. D-gluconate + NAD⁺ = 5-dehydro-D-gluconate + NADH + H⁺

D-gluconate (Bound ligand (Het Group name = TLA) matches with 64.29% similarity) + NADP(+) (Bound ligand (Het Group name = NAP) corresponds exactly) = 5-dehydro-D-gluconate + NADPH + H(+)

D-gluconate (Bound ligand (Het Group name = TLA) matches with 64.29% similarity) + NAD(+) (Bound ligand (Het Group name = NAP) matches with 91.67% similarity) = 5-dehydro-D-gluconate + NADH + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site