PDBsum entry 5ejh

Transferase

PDB id: 5ejh

Contents

Protein chain

259 a.a.

Ligands

NAP

Waters ×146

PDB id:

5ejh

Name:

Transferase

Title:

Crystal structure of NAD kinase v98s mutant from listeria monocytogenes

Structure:

NAD kinase 1. Chain: a. Synonym: atp-dependent NAD kinase. Engineered: yes

Source:

Listeria monocytogenes. Organism_taxid: 1639. Gene: nadk1, lmo0968. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

2.00Å R-factor: 0.181 R-free: 0.233

Authors:

G.Poncet-Montange,L.Assairi,M.Gelin,S.Pochet,G.Labesse

Key ref:

G.Poncet-Montange et al. Crystal structure of NAD kinase 1 from listeria monocytogenes: V98s mutant. To be published, .

Date:

01-Nov-15 Release date: 09-Nov-16

Protein chain

Q8Y8D7  (NADK1_LISMO) -  NAD kinase 1 from Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)

Seq: 264 a.a.

Struc: 259 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.2.7.1.23 - NAD(+) kinase.
• Reaction: NAD⁺ + ATP = ADP + NADP⁺ + H⁺

NAD(+) + ATP = ADP + NADP(+) (Bound ligand (Het Group name = NAP) corresponds exactly) + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site