PDBsum entry 5eim

RNA binding protein/RNA

PDB id: 5eim

Contents

Protein chains

157 a.a.

DNA/RNA

Waters ×365

PDB id:

5eim

Name:

RNA binding protein/RNA

Title:

Yth domain-containing protein mmi1 and RNA complex

Structure:

Yth domain-containing protein mmi1. Chain: a, b. Fragment: unp residues 326-488. Synonym: yth domain of spmmi1, meiotic mRNA interception protein 1. Engineered: yes. RNA (5'-r( Ap Up Up Ap Ap Ap Cp A)-3'). Chain: c, d. Engineered: yes

Source:

Schizosaccharomyces pombe 972h-. Fission yeast. Organism_taxid: 284812. Strain: 972h-. Gene: mmi1, spcc736.12c. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Synthetic construct.

Resolution:

1.54Å R-factor: 0.174 R-free: 0.225

Authors:

B.X.Wu,J.H.Xu,S.C.Su,J.B.Ma

Key ref:

B.Wu et al. (2017). Structural insights into the specific recognition of DSR by the YTH domain containing protein Mmi1. Biochem Biophys Res Commun, 491, 310-316. PubMed id: 28735863 DOI: 10.1016/j.bbrc.2017.07.104

Date:

30-Oct-15 Release date: 27-Jan-16

Protein chains

O74958  (MMI1_SCHPO) -  RNA binding exosome specificity factor Mmi1 from Schizosaccharomyces pombe (strain 972 / ATCC 24843)

Seq: 488 a.a.

Struc: 157 a.a.

DNA/RNA chains

A-U-U-A-A-A-C-A 8 bases

A-U-U-A-A-A-C-A 8 bases

DOI no: 10.1016/j.bbrc.2017.07.104

Biochem Biophys Res Commun 491:310-316 (2017)

PubMed id: 28735863

Structural insights into the specific recognition of DSR by the YTH domain containing protein Mmi1.

B.Wu, J.Xu, S.Su, H.Liu, J.Gan, J.Ma.

ABSTRACT

Meiosis is one of the most dramatic differentiation programs accompanied by a striking change in gene expression profiles in fission yeast Schizosaccharomyces pombe. Whereas a number of meiosis-specific transcripts are expressed untimely in mitotic cells, and the entry of meiosis will be blocked as the accumulation of meiosis-specific mRNAs in the mitotic cells. A YTH domain containing protein Mmi1 was identified as a pivotal effector in a post-transcriptional event termed selective elimination of meiosis-specific mRNAs. Mmi1 can recognize and bind a class of meiosis-specific transcripts expressed inappropriately in mitotic cells, which all contain a conservative region called DSR, as a mark to remove them in cooperation with nuclear exosomes. Here we report the 1.6 Å resolution crystal structure of the Mmi1-YTH domain in complex with a high consensus hexanucleotide motif, which is multiple copied in the DSR region. Our structure observations, supported by site-directed mutations of key residues illustrate the mechanism for specific recognition of DSR-RNA by Mmi1. Moreover, different from other YTH domain family proteins, Mmi1-YTH domain has a distinctive RNA-binding properties although it has a similar fold as other ones.