PDBsum entry 5eeo

Oxidoreductase

PDB id: 5eeo

Contents

Protein chain

806 a.a.

Metals

FE2

Waters ×317

PDB id:

5eeo

Name:

Oxidoreductase

Title:

Soybean lipoxygenase(l1)-t756r

Structure:

Seed linoleate 13s-lipoxygenase-1. Chain: a. Synonym: lipoxygenase-1,l-1. Engineered: yes. Mutation: yes

Source:

Glycine max. Soybean. Organism_taxid: 3847. Gene: lox1.1, lox1. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

2.10Å R-factor: 0.189 R-free: 0.254

Authors:

B.Mikami,T.Nagaya,Y.Hioki

Key ref:

B.Mikami et al. The structural basis for specificity in lipoxygenase catalysis. To be published, .

Date:

23-Oct-15 Release date: 25-Nov-15

Protein chain

P08170  (LOX1_SOYBN) -  Seed linoleate 13S-lipoxygenase-1 from Glycine max

Seq: 839 a.a.

Struc: 806 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.1.13.11.12 - linoleate 13S-lipoxygenase.
• Reaction:
  1. (9Z,12Z)-octadecadienoate + O2 = (13S)-hydroperoxy-(9Z,11E)- octadecadienoate
  2. (9Z,12Z,15Z)-octadecatrienoate + O2 = (13S)-hydroperoxy-(9Z,11E,15Z)- octadecatrienoate
• Cofactor: Fe cation

Molecule diagrams generated from .mol files obtained from the KEGG ftp site