PDBsum entry 5e3i

Ligase

PDB id: 5e3i

Contents

Protein chains

388 a.a.

Ligands

ATP ×2

HIS ×2

Metals

_MG ×2

Waters ×460

PDB id:

5e3i

Name:

Ligase

Title:

Crystal structure of a histidyl-tRNA synthetase from acinetobacter baumannii with bound l-histidine and atp

Structure:

Histidine--tRNA ligase. Chain: a, b. Synonym: histidyl-tRNA synthetase. Engineered: yes

Source:

Acinetobacter baumannii. Organism_taxid: 470. Gene: hiss, abuw_3376, iomtu433_3293, ru84_15845. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

2.20Å R-factor: 0.166 R-free: 0.205

Authors:

Seattle Structural Genomics Center For Infectious Disease (Ssgcid)

Key ref:

Ssgcid et al. Crystal structure of a histidyl-Trna synthetase from acinetobacter baumannii with bound l-Histidine and at. To be published, .

Date:

02-Oct-15 Release date: 04-Nov-15

Protein chains

B0VKR7  (SYH_ACIBS) -  Histidine--tRNA ligase from Acinetobacter baumannii (strain SDF)

Seq: 430 a.a.

Struc: 388 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.6.1.1.21 - histidine--tRNA ligase.
• Reaction: tRNA(His) + L-histidine + ATP = L-histidyl-tRNA(His) + AMP + diphosphate + H⁺

tRNA(His) + L-histidine (Bound ligand (Het Group name = HIS) corresponds exactly) + ATP (Bound ligand (Het Group name = ATP) corresponds exactly) = L-histidyl-tRNA(His) + AMP + diphosphate + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site