PDBsum entry 5cws

Protein transport

PDB id: 5cws

Contents

Protein chains

208 a.a.

223 a.a.

169 a.a.

180 a.a.

241 a.a.

40 a.a.

Metals

_OS ×2

PDB id:

5cws

Name:

Protein transport

Title:

Crystal structure of the intact chaetomium thermophilum nsp1-nup49- nup57 channel nucleoporin heterotrimer bound to its nic96 nuclear pore complex attachment site

Structure:

Sab-158 fab light chain. Chain: a, g. Engineered: yes. Sab-158 fab heavy chain. Chain: b, h. Engineered: yes. Nucleoporin nsp1. Chain: c, i. Fragment: unp residues 467-674.

Source:

Homo sapiens. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 562. Chaetomium thermophilum (strain dsm 1495 / cbs 144.50 / imi 039719). Organism_taxid: 759272. Strain: dsm 1495 / cbs 144.50 / imi 039719. Gene: nsp1, ctht_0054390.

Resolution:

3.77Å R-factor: 0.231 R-free: 0.265

Authors:

C.J.Bley,S.Petrovic,M.Paduch,V.Lu,A.A.Kossiakoff,A.Hoelz

Key ref:

T.Stuwe et al. (2015). Architecture of the fungal nuclear pore inner ring complex. Science, 350, 56-64. PubMed id: 26316600 DOI: 10.1126/science.aac9176

Date:

28-Jul-15 Release date: 14-Oct-15

Protein chains

No UniProt id for this chain

Struc: 208 a.a.

Protein chains

No UniProt id for this chain

Struc: 223 a.a.

Protein chains

G0SBQ3  (NSP1_CHATD) -  Nucleoporin NSP1 from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)

Seq: 678 a.a.

Struc: 169 a.a.

Protein chains

G0S4X2  (NUP49_CHATD) -  Nucleoporin NUP49 from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)

Seq: 470 a.a.

Struc: 180 a.a.*

Protein chains

G0S0R2  (NUP57_CHATD) -  Nucleoporin NUP57 from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)

Seq: 326 a.a.

Struc: 241 a.a.

Protein chains

G0S024  (NIC96_CHATD) -  Nucleoporin NIC96 from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)

Seq: 1112 a.a.

Struc: 40 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

DOI no: 10.1126/science.aac9176

Science 350:56-64 (2015)

PubMed id: 26316600

Architecture of the fungal nuclear pore inner ring complex.

T.Stuwe, C.J.Bley, K.Thierbach, S.Petrovic, S.Schilbach, D.J.Mayo, T.Perriches, E.J.Rundlet, Y.E.Jeon, L.N.Collins, F.M.Huber, D.H.Lin, M.Paduch, A.Koide, V.Lu, J.Fischer, E.Hurt, S.Koide, A.A.Kossiakoff, A.Hoelz.

ABSTRACT

The nuclear pore complex (NPC) constitutes the sole gateway for bidirectional nucleocytoplasmic transport. We present the reconstitution and interdisciplinary analyses of the ~425-kilodalton inner ring complex (IRC), which forms the central transport channel and diffusion barrier of the NPC, revealing its interaction network and equimolar stoichiometry. The Nsp1•Nup49•Nup57 channel nucleoporin heterotrimer (CNT) attaches to the IRC solely through the adaptor nucleoporin Nic96. The CNT•Nic96 structure reveals that Nic96 functions as an assembly sensor that recognizes the three-dimensional architecture of the CNT, thereby mediating the incorporation of a defined CNT state into the NPC. We propose that the IRC adopts a relatively rigid scaffold that recruits the CNT to primarily form the diffusion barrier of the NPC, rather than enabling channel dilation.