PDBsum entry 5cu7

Hydrolase

PDB id: 5cu7

Contents

Protein chains

396 a.a.

Ligands

PO4 ×2

Waters ×571

PDB id:

5cu7

Name:

Hydrolase

Title:

Bacteroides thetaiotaomicron multiple inositol polyphosphate phosphatase a324d mutant

Structure:

Multiple inositol polyphosphate histidine phosphatase 1. Chain: a, b. Synonym: minpp. Engineered: yes

Source:

Bacteroides thetaiotaomicron vpi-5482. Organism_taxid: 226186. Gene: bt_4744. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Expression_system_variant: rosetta2 plyss.

Resolution:

1.73Å R-factor: 0.157 R-free: 0.181

Authors:

A.W.H.Li,A.M.Hemmings

Key ref:

A.W.H.Li et al. The hydrolytic positional specificity towards phytic a bacterial multiple inositol polyphosphate phosphata. To be published, .

Date:

24-Jul-15 Release date: 17-Aug-16

Protein chains

Q89YI8  (Q89YI8_BACTN) -  Multiple inositol polyphosphate histidine phosphatase 1 from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50)

Seq: 425 a.a.

Struc: 396 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class 1: E.C.3.1.3.62 - multiple inositol-polyphosphate phosphatase.
• Reaction:
  1. 1D-myo-inositol hexakisphosphate + H2O = 1D-myo-inositol 1,2,4,5,6- pentakisphosphate + phosphate
  2. 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + H2O = 1D-myo-inositol 1,2,5,6-tetrakisphosphate + phosphate
  3. 1D-myo-inositol 1,2,5,6-tetrakisphosphate + H2O = 1D-myo-inositol 1,2,6-trisphosphate + phosphate
  4. 1D-myo-inositol 1,2,6-trisphosphate + H2O = 1D-myo-inositol 1,2- bisphosphate + phosphate
  5. 1D-myo-inositol 1,2-bisphosphate + H2O = 1D-myo-inositol 2-phosphate + phosphate

1D-myo-inositol hexakisphosphate + H2O = 1D-myo-inositol 1,2,4,5,6- pentakisphosphate + phosphate (Bound ligand (Het Group name = PO4) corresponds exactly)

1D-myo-inositol 1,2,4,5,6-pentakisphosphate + H2O = 1D-myo-inositol 1,2,5,6-tetrakisphosphate + phosphate (Bound ligand (Het Group name = PO4) corresponds exactly)

1D-myo-inositol 1,2,5,6-tetrakisphosphate + H2O = 1D-myo-inositol 1,2,6-trisphosphate + phosphate (Bound ligand (Het Group name = PO4) corresponds exactly)

1D-myo-inositol 1,2,6-trisphosphate + H2O = 1D-myo-inositol 1,2- bisphosphate + phosphate (Bound ligand (Het Group name = PO4) corresponds exactly)

1D-myo-inositol 1,2-bisphosphate + H2O = 1D-myo-inositol 2-phosphate + phosphate (Bound ligand (Het Group name = PO4) corresponds exactly)

• Enzyme class 2: E.C.3.1.3.80 - 2,3-bisphosphoglycerate 3-phosphatase.
• Reaction: (2R)-2,3-bisphosphoglycerate + H2O = (2R)-2-phosphoglycerate + phosphate

(2R)-2,3-bisphosphoglycerate + H2O = (2R)-2-phosphoglycerate + phosphate (Bound ligand (Het Group name = PO4) corresponds exactly)

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site