PDBsum entry 5cpi

Structural protein/DNA

PDB id: 5cpi

Contents

Protein chains

97 a.a.

78 a.a.

108 a.a.

93 a.a.

83 a.a.

DNA/RNA

PDB id:

5cpi

Name:

Structural protein/DNA

Title:

Nucleosome containing unmethylated sat2r DNA

Structure:

Histone h3.1. Chain: a, e. Synonym: histone h3/a,histone h3/b,histone h3/c,histone h3/d,histone h3/f,histone h3/h,histone h3/i,histone h3/j,histone h3/k,histone h3/l. Engineered: yes. Histone h4. Chain: b, f. Engineered: yes.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: hist1h3a, h3fa, hist1h3b, h3fl, hist1h3c, h3fc, hist1h3d, h3fb, hist1h3e, h3fd, hist1h3f, h3fi, hist1h3g, h3fh, hist1h3h, h3fk, hist1h3i, h3ff, hist1h3j, h3fj. Expressed in: escherichia coli. Expression_system_taxid: 469008. Gene: hist1h4a, h4/a, h4fa, hist1h4b, h4/i, h4fi, hist1h4c, h4/g,

Resolution:

2.90Å R-factor: 0.253 R-free: 0.295

Authors:

A.Osakabe,Y.Arimura,F.Adachi,K.Maehara,Y.Ohkawa,H.Kurumizaka

Key ref:

A.Osakabe et al. (2015). Influence of DNA methylation on positioning and DNA flexibility of nucleosomes with pericentric satellite. Open biology, 5, . PubMed id: 26446621

Date:

21-Jul-15 Release date: 28-Oct-15

Protein chains

P68431  (H31_HUMAN) -  Histone H3.1 from Homo sapiens

Seq: 136 a.a.

Struc: 97 a.a.

Protein chain

P62805  (H4_HUMAN) -  Histone H4 from Homo sapiens

Seq: 103 a.a.

Struc: 78 a.a.

Protein chains

P04908  (H2A1B_HUMAN) -  Histone H2A type 1-B/E from Homo sapiens

Seq: 130 a.a.

Struc: 108 a.a.

Protein chains

P06899  (H2B1J_HUMAN) -  Histone H2B type 1-J from Homo sapiens

Seq: 126 a.a.

Struc: 93 a.a.

Protein chain

P62805  (H4_HUMAN) -  Histone H4 from Homo sapiens

Seq: 103 a.a.

Struc: 83 a.a.

DNA/RNA chains

A-T-C-C-A-A-A-T-G-G-A-T-T-C-G-A-A-T-G-G-A-A-T-C-A-T-T-G-A-A-T-G-G-A-A-A-T-G-A- 146 bases

A-T-C-A-G-A-T-T-C-C-A-T-T-C-G-A-A-T-C-C-A-T-T-C-G-A-A-A-A-T-G-A-T-T-A-C-A-T-T- 146 bases