PDBsum entry 5axs

Metal binding protein

PDB id: 5axs

Contents

Protein chain

174 a.a.

Ligands

4LF

SO4

GOL ×3

Metals

_CD ×5

Waters ×152

PDB id:

5axs

Name:

Metal binding protein

Title:

Crystal structure of cdcat-fn

Structure:

Ferritin light chain. Chain: a. Synonym: ferritin l subunit. Engineered: yes. Mutation: yes

Source:

Equus caballus. Horse. Organism_taxid: 9796. Gene: ftl. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.67Å R-factor: 0.163 R-free: 0.188

Authors:

S.Abe,H.Nakajima,M.Kondo,T.Nakane,T.Nakao,T.Ueno,Y.Watanabe

Key ref:

H.Nakajima et al. (2015). Construction of an enterobactin analogue with symmetrically arranged monomer subunits of ferritin. Chem Commun (camb), 51, 16609-16612. PubMed id: 26404005 DOI: 10.1039/c5cc06904a

Date:

01-Aug-15 Release date: 18-Nov-15

Protein chain

P02791  (FRIL_HORSE) -  Ferritin light chain from Equus caballus

Seq: 175 a.a.

Struc: 174 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

DOI no: 10.1039/c5cc06904a

Chem Commun (camb) 51:16609-16612 (2015)

PubMed id: 26404005

Construction of an enterobactin analogue with symmetrically arranged monomer subunits of ferritin.

H.Nakajima, M.Kondo, T.Nakane, S.Abe, T.Nakao, Y.Watanabe, T.Ueno.

ABSTRACT

A set of three catecholamide ligands mimicking the structure of enterobactin was constructed on ferritin, where the 3-fold symmetric arrangement of the monomer subunits served as a foundation to form a coordination space. Similar to enterobactin, the ligands showed strong affinity for the ferric ion and formed a tris-catechoyl complex. Crystallography revealed that the complex was embedded in the entrance of the 3-fold axis channel.