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PDBsum entry 4zzc
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Membrane protein, transport protein
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PDB id
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4zzc
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PDB id:
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| Name: |
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Membrane protein, transport protein
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Title:
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The glic pentameric ligand-gated ion channel open form complexed to xenon
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Structure:
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Proton-gated ion channel. Chain: a, b, c, d, e. Synonym: glic,ligand-gated ion channel,lgic, glic pentameric ligand- gated ion channel. Engineered: yes
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Source:
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Gloeobacter violaceus (strain pcc 7421). Organism_taxid: 251221. Strain: pcc 7421. Gene: glvi, glr4197. Expressed in: escherichia coli. Expression_system_taxid: 562
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Resolution:
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3.10Å
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R-factor:
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0.209
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R-free:
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0.228
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Authors:
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L.Sauguet,Z.Fourati,T.Prange,M.Delarue,N.Colloc'H
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Key ref:
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L.Sauguet
et al.
(2016).
Structural Basis for Xenon Inhibition in a Cationic Pentameric Ligand-Gated Ion Channel.
Plos One,
11,
e0149795.
PubMed id:
DOI:
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Date:
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22-May-15
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Release date:
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02-Mar-16
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PROCHECK
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Headers
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References
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Q7NDN8
(GLIC_GLOVI) -
Proton-gated ion channel from Gloeobacter violaceus (strain ATCC 29082 / PCC 7421)
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Seq:
Struc:
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359 a.a.
311 a.a.
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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DOI no:
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Plos One
11:e0149795
(2016)
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PubMed id:
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Structural Basis for Xenon Inhibition in a Cationic Pentameric Ligand-Gated Ion Channel.
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L.Sauguet,
Z.Fourati,
T.Prangé,
M.Delarue,
N.Colloc'h.
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ABSTRACT
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GLIC receptor is a bacterial pentameric ligand-gated ion channel whose action is
inhibited by xenon. Xenon has been used in clinical practice as a potent gaseous
anaesthetic for decades, but the molecular mechanism of interactions with its
integral membrane receptor targets remains poorly understood. Here we
characterize by X-ray crystallography the xenon-binding sites within both the
open and "locally-closed" (inactive) conformations of GLIC. Major
binding sites of xenon, which differ between the two conformations, were
identified in three distinct regions that all belong to the trans-membrane
domain of GLIC: 1) in an intra-subunit cavity, 2) at the interface between
adjacent subunits, and 3) in the pore. The pore site is unique to the
locally-closed form where the binding of xenon effectively seals the channel. A
putative mechanism of the inhibition of GLIC by xenon is proposed, which might
be extended to other pentameric cationic ligand-gated ion channels.
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Links
