PDBsum entry 4ztt

Oxidoreductase

PDB id: 4ztt

Contents

Protein chains

(+ 0 more) 162 a.a.

Ligands

FEO-_OH-_OH ×2

GOL ×14

OXY

_OH ×4

PER

FEO-_OH

__O-__O

Metals

FE2 ×2

_FE ×5

Waters ×883

PDB id:

4ztt

Name:

Oxidoreductase

Title:

Crystal structures of ferritin mutants reveal diferric-peroxo intermediates

Structure:

Bacterial non-heme ferritin. Chain: a, b, c, d, e, f. Synonym: ferritin-1. Engineered: yes. Mutation: yes

Source:

Escherichia coli dh1. Organism_taxid: 536056. Gene: ftna, z2960, ecs2613. Expressed in: escherichia coli 'bl21-gold(de3)plyss ag'. Expression_system_taxid: 866768

Resolution:

1.83Å R-factor: 0.158 R-free: 0.192

Authors:

S.Kim,Y.H.Park,S.W.Jung,J.H.Seok,Y.B.Chung,D.B.Lee,G.Gowda,J.H.Lee, H.R.Han,A.E.Cho,C.Lee,M.S.Chung,K.H.Kim

Key ref:

S.Kim et al. (2016). Structural Basis of Novel Iron-Uptake Route and Reaction Intermediates in Ferritins from Gram-Negative Bacteria. J Mol Biol, 428, 5007-5018. PubMed id: 27777002 DOI: 10.1016/j.jmb.2016.10.022

Date:

15-May-15 Release date: 15-Jun-16

Protein chains

P0A998  (FTNA_ECOLI) -  Bacterial non-heme ferritin from Escherichia coli (strain K12)

Seq: 165 a.a.

Struc: 164 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.1.16.3.2 - bacterial non-heme ferritin.
• Reaction: 4 Fe²⁺ + O2 + 6 H2O = 4 iron(III) oxide-hydroxide + 12 H⁺

4 × Fe(2+) + O2 (Bound ligand (Het Group name = OXY) corresponds exactly) + 6 × H2O = 4 × iron(III) oxide-hydroxide + 12 × H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Key reference

DOI no: 10.1016/j.jmb.2016.10.022

J Mol Biol 428:5007-5018 (2016)

PubMed id: 27777002

Structural Basis of Novel Iron-Uptake Route and Reaction Intermediates in Ferritins from Gram-Negative Bacteria.

S.Kim, J.H.Lee, J.H.Seok, Y.H.Park, S.W.Jung, A.E.Cho, C.Lee, M.S.Chung, K.H.Kim.

ABSTRACT

No abstract given.