PDBsum entry 4zor

Virus

PDB id: 4zor

Contents

Protein chains

128 a.a.

Ligands

PO4 ×4

Waters ×432

PDB id:

4zor

Name:

Virus

Title:

The structure of the s37p ms2 viral capsid assembly.

Structure:

Coat protein. Chain: a, b, c, d, e. Engineered: yes. Mutation: yes

Source:

Enterobacteria phage ms2. Organism_taxid: 329852. Expressed in: escherichia coli k-12. Expression_system_taxid: 83333

Resolution:

2.20Å R-factor: 0.219 R-free: 0.243

Authors:

M.A.Asensio,B.Sankaran,P.H.Zwart,D.Tullman-Ercek

Key ref:

M.A.Asensio et al. (2016). A Selection for Assembly Reveals That a Single Amino Acid Mutant of the Bacteriophage MS2 Coat Protein Forms a Smaller Virus-like Particle. Nano Lett, 16, 5944-5950. PubMed id: 27549001 DOI: 10.1021/acs.nanolett.6b02948

Date:

06-May-15 Release date: 07-Sep-16

Protein chains

P03612  (CAPSD_BPMS2) -  Capsid protein from Escherichia phage MS2

Seq: 130 a.a.

Struc: 128 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

DOI no: 10.1021/acs.nanolett.6b02948

Nano Lett 16:5944-5950 (2016)

PubMed id: 27549001

A Selection for Assembly Reveals That a Single Amino Acid Mutant of the Bacteriophage MS2 Coat Protein Forms a Smaller Virus-like Particle.

M.A.Asensio, N.M.Morella, C.M.Jakobson, E.C.Hartman, J.E.Glasgow, B.Sankaran, P.H.Zwart, D.Tullman-Ercek.

ABSTRACT

Virus-like particles are used to encapsulate drugs, imaging agents, enzymes, and other biologically active molecules in order to enhance their function. However, the size of most virus-like particles is inflexible, precluding the design of appropriately sized containers for different applications. Here, we describe a chromatographic selection for virus-like particle assembly. Using this selection, we identified a single amino acid substitution to the coat protein of bacteriophage MS2 that mediates a uniform switch in particle geometry from T = 3 to T = 1 icosahedral symmetry. The resulting smaller particle retains the ability to be disassembled and reassembled in vitro and to be chemically modified to load cargo into its interior cavity. The pair of 27 and 17 nm MS2 particles will allow direct examination of the effect of size on function in established applications of virus-like particles, including drug delivery and imaging.