PDBsum entry 4zmm

Transferase

PDB id: 4zmm

Contents

Protein chains

164 a.a.

Ligands

C2E ×2

Waters ×69

PDB id:

4zmm

Name:

Transferase

Title:

Ggdef domain of dcsbis complexed with c-di-gmp

Structure:

Diguanylate cyclase. Chain: a, b. Fragment: unp residues 123-341. Engineered: yes

Source:

Pseudomonas aeruginosa pao1. Organism_taxid: 208964. Strain: pao1. Gene: pa2771. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

2.50Å R-factor: 0.204 R-free: 0.248

Authors:

Y.Chen,C.Liu,S.Liu,K.Chi,L.Gu

Key ref:

Y.Chen et al. Crystal structure of dcsbis ggdef domain complexed wi c-Di-Gmp. To be published, .

Date:

04-May-15 Release date: 04-May-16

Protein chains

Q9I072  (Q9I072_PSEAE) -  diguanylate cyclase from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

Seq: 341 a.a.

Struc: 164 a.a.

Enzyme reactions

• Enzyme class: E.C.2.7.7.65 - diguanylate cyclase.
• Reaction: 2 GTP = 3',3'-c-di-GMP + 2 diphosphate

2 × GTP (Bound ligand (Het Group name = C2E) matches with 44.44% similarity) = 3',3'-c-di-GMP + 2 × diphosphate

• Cofactor: Mg(2+); Mn(2+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site