PDBsum entry 4zmf

Transferase

PDB id: 4zmf

Contents

Protein chains

251 a.a.

Ligands

AMP ×2

PO4 ×2

Metals

_ZN ×2

Waters ×192

PDB id:

4zmf

Name:

Transferase

Title:

Phosphorylated aspartate in the crystal structure of the alpha-kinase domain of myosin-ii heavy chain kinase a

Structure:

Myosin heavy chain kinase a. Chain: a, b. Fragment: residues 552-841. Synonym: mhck-a. Engineered: yes

Source:

Dictyostelium discoideum. Slime mold. Organism_taxid: 44689. Gene: mhka, mhcka, ddb_g0291231. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

2.39Å R-factor: 0.195 R-free: 0.217

Authors:

Q.Ye,Z.Jia

Key ref:

Y.Yang et al. (2015). Characterization of the Catalytic and Nucleotide Binding Properties of the α-Kinase Domain of Dictyostelium Myosin-II Heavy Chain Kinase A. J Biol Chem, 290, 23935-23946. PubMed id: 26260792 DOI: 10.1074/jbc.M115.672410

Date:

04-May-15 Release date: 19-Aug-15

Protein chains

P42527  (MHCKA_DICDI) -  Myosin heavy chain kinase A from Dictyostelium discoideum

Seq: 1146 a.a.

Struc: 251 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.2.7.11.7 - [myosin heavy-chain] kinase.
• Reaction: L-threonyl-[myosin heavy-chain] + ATP = O-phospho-L-threonyl-[myosin heavy-chain] + ADP + H⁺

L-threonyl-[myosin heavy-chain] + ATP = O-phospho-L-threonyl-[myosin heavy-chain] (Bound ligand (Het Group name = AMP) matches with 85.19% similarity) + ADP + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

DOI no: 10.1074/jbc.M115.672410

J Biol Chem 290:23935-23946 (2015)

PubMed id: 26260792

Characterization of the Catalytic and Nucleotide Binding Properties of the α-Kinase Domain of Dictyostelium Myosin-II Heavy Chain Kinase A.

Y.Yang, Q.Ye, Z.Jia, G.P.Côté.

ABSTRACT

No abstract given.