PDBsum entry 4zme

Transferase

PDB id: 4zme

Contents

Protein chains

262 a.a.

Ligands

ADN ×2

PO4 ×4

PGE

Metals

_ZN ×2

Waters ×459

PDB id:

4zme

Name:

Transferase

Title:

Crystal structure of the alpha-kinase domain of myosin-ii heavy chain kinase a in complex with adenosine

Structure:

Myosin heavy chain kinase a. Chain: a, b. Fragment: residues 552-841. Synonym: mhck-a. Engineered: yes

Source:

Dictyostelium discoideum. Slime mold. Organism_taxid: 44689. Gene: mhka, mhcka, ddb_g0291231. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

1.98Å R-factor: 0.215 R-free: 0.236

Authors:

Q.Ye,Z.Jia

Key ref:

Y.Yang et al. (2015). Characterization of the Catalytic and Nucleotide Binding Properties of the α-Kinase Domain of Dictyostelium Myosin-II Heavy Chain Kinase A. J Biol Chem, 290, 23935-23946. PubMed id: 26260792 DOI: 10.1074/jbc.M115.672410

Date:

03-May-15 Release date: 19-Aug-15

Protein chains

P42527  (MHCKA_DICDI) -  Myosin heavy chain kinase A from Dictyostelium discoideum

Seq: 1146 a.a.

Struc: 262 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.2.7.11.7 - [myosin heavy-chain] kinase.
• Reaction: L-threonyl-[myosin heavy-chain] + ATP = O-phospho-L-threonyl-[myosin heavy-chain] + ADP + H⁺

L-threonyl-[myosin heavy-chain] + ATP = O-phospho-L-threonyl-[myosin heavy-chain] (Bound ligand (Het Group name = ADN) matches with 70.37% similarity) + ADP + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

DOI no: 10.1074/jbc.M115.672410

J Biol Chem 290:23935-23946 (2015)

PubMed id: 26260792

Characterization of the Catalytic and Nucleotide Binding Properties of the α-Kinase Domain of Dictyostelium Myosin-II Heavy Chain Kinase A.

Y.Yang, Q.Ye, Z.Jia, G.P.Côté.

ABSTRACT

No abstract given.