PDBsum entry 4zkj

Unknown function

PDB id: 4zkj

Contents

Protein chains

285 a.a.

Ligands

GOL ×6

Waters ×249

PDB id:

4zkj

Name:

Unknown function

Title:

Crystal structure of crispr-associated protein

Structure:

Crispr-associated protein cas1. Chain: a, b. Fragment: unp residues 2-289. Engineered: yes

Source:

Streptococcus pyogenes m1 476. Organism_taxid: 1207470. Gene: cas1, m1gas476_0831. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

2.25Å R-factor: 0.183 R-free: 0.211

Authors:

D.Ka,E.Bae

Key ref:

D.Ka et al. (2016). Crystal Structure of Streptococcus pyogenes Cas1 and Its Interaction with Csn2 in the Type II CRISPR-Cas System. Structure, 24, 70-79. PubMed id: 26671707 DOI: 10.1016/j.str.2015.10.019

Date:

30-Apr-15 Release date: 13-Jan-16

Protein chains

J7M1H5  (J7M1H5_STRP1) -

Enzyme reactions

• Enzyme class: E.C.3.1.-.-

DOI no: 10.1016/j.str.2015.10.019

Structure 24:70-79 (2016)

PubMed id: 26671707

Crystal Structure of Streptococcus pyogenes Cas1 and Its Interaction with Csn2 in the Type II CRISPR-Cas System.

D.Ka, H.Lee, Y.D.Jung, K.Kim, C.Seok, N.Suh, E.Bae.

ABSTRACT

CRISPRs and Cas proteins constitute an RNA-guided microbial immune system against invading nucleic acids. Cas1 is a universal Cas protein found in all three types of CRISPR-Cas systems, and its role is implicated in new spacer acquisition during CRISPR-mediated adaptive immunity. Here, we report the crystal structure of Streptococcus pyogenes Cas1 (SpCas1) in a type II CRISPR-Cas system and characterize its interaction with S. pyogenes Csn2 (SpCsn2). The SpCas1 structure reveals a unique conformational state distinct from type I Cas1 structures, resulting in a more extensive dimerization interface, a more globular overall structure, and a disruption of potential metal-binding sites for catalysis. We demonstrate that SpCas1 directly interacts with SpCsn2, and identify the binding interface and key residues for Cas complex formation. These results provide structural information for a type II Cas1 protein, and lay a foundation for studying multiprotein Cas complexes functioning in type II CRISPR-Cas systems.