 |
PDBsum entry 4zk0
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Immune system
|
PDB id
|
|
|
|
4zk0
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
PDB id:
|
|
|
|
| Name: |
|
Immune system
|
|
|
Title:
|
|
Psoriasis pathogenesis - pso p27 constitute a compact structure forming large aggregates. High ph structure
|
|
Structure:
|
|
Serpin b4. Chain: a. Synonym: leupin,peptidase inhibitor 11,pi-11,squamous cell carcinoma antigen 2,scca-2. Engineered: yes
|
|
Source:
|
|
Homo sapiens. Human. Organism_taxid: 9606. Gene: serpinb4, pi11, scca2. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Expression_system_variant: ripl
|
|
Resolution:
|
|
|
2.15Å
|
R-factor:
|
0.177
|
R-free:
|
0.234
|
|
|
Authors:
|
|
R.Helland,H.Lysvand,G.Slupphaug,O.J.Iversen
|
|
Key ref:
|
|
H.Lysvand
et al.
(2015).
Psoriasis pathogenesis - Pso p27 constitutes a compact structure forming large aggregates.
Biochem Biophys Rep,
2,
132-136.
PubMed id:
|
|
|
Date:
|
|
|
29-Apr-15
|
Release date:
|
01-Jul-15
|
|
|
|
|
|
|
PROCHECK
|
|
|
|
|
|
Headers
|
|
|
|
References
|
|
|
|
|
|
|
|
P29508
(SPB3_HUMAN) -
Serpin B3 from Homo sapiens
|
|
|
|
Seq:
Struc:
|
|
|
|
390 a.a.
367 a.a.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Key: |
 |
PfamA domain |
|
|
 |
Secondary structure |
|
 |
CATH domain |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
| |
|
|
Biochem Biophys Rep
2:132-136
(2015)
|
|
PubMed id:
|
|
|
|
|
|
| |
|
Psoriasis pathogenesis - Pso p27 constitutes a compact structure forming large aggregates.
|
|
H.Lysvand,
R.Helland,
L.Hagen,
G.Slupphaug,
O.J.Iversen.
|
|
|
|
|
| |
ABSTRACT
|
|
|
|
| |
|
|
Psoriasis is a chronic inflammatory skin disease. The absence of microbial
organisms as potential causal agents has given rise to the hypothesis that the
inflammation is due to an autoimmune reaction. The defined inflamed areas of the
skin lesions argue for an immunological disease with a local production of a
causal antigen. Pso p27 is a protein generated in mast cells in psoriatic
plaques, but not in uninvolved skin. We recently demonstrated that the Pso p27
is generated by cleavage of SerpinB3 (SCCA1) in the presence of mast cell
associated chymase. In this communication we demonstrate by X-ray
crystallographic analysis that the cleavage products associate into a complex
similar to SCCA1, but with the reactive centre loop inserted into a 5-stranded
central β-sheet. Native gel electrophoresis show that these Pso p27 complexes
form large aggregates which may be of significance with respect to an
immunogenic role of Pso p27.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
 |
 |
|
Links
