PDBsum entry 4ziz

Electron transport

PDB id: 4ziz

Contents

Protein chains

162 a.a.

172 a.a.

Ligands

CYC ×3

Waters ×166

PDB id:

4ziz

Name:

Electron transport

Title:

Serial femtosecond crystallography of soluble proteins in lipidic cubic phase (c-phycocyanin from t. Elongatus)

Structure:

C-phycocyanin alpha chain. Chain: a. C-phycocyanin beta chain. Chain: b

Source:

Thermosynechococcus elongatus (strain bp-1). Organism_taxid: 197221. Strain: bp-1. Strain: bp-1

Resolution:

1.75Å R-factor: 0.206 R-free: 0.254

Authors:

R.Fromme,A.Ishchenko,M.Metz,S.Roy-Chowdhury,S.Basu,S.Boutet,P.Fromme, T.A.White,A.Barty,J.C.H.Spence,U.Weierstall,W.Liu,V.Cherezov

Key ref:

R.Fromme et al. (2015). Serial femtosecond crystallography of soluble proteins in lipidic cubic phase. Iucrj, 2, 545-551. PubMed id: 26306196 DOI: 10.1107/S2052252515013160

Date:

28-Apr-15 Release date: 19-Aug-15

Protein chain

P50032  (PHCA_THEEB) -  C-phycocyanin alpha subunit from Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1)

Seq: 162 a.a.

Struc: 162 a.a.

Protein chain

P50033  (PHCB_THEEB) -  C-phycocyanin beta subunit from Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1)

Seq: 172 a.a.

Struc: 172 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chains A, B: E.C.?

DOI no: 10.1107/S2052252515013160

Iucrj 2:545-551 (2015)

PubMed id: 26306196

Serial femtosecond crystallography of soluble proteins in lipidic cubic phase.

R.Fromme, A.Ishchenko, M.Metz, S.R.Chowdhury, S.Basu, S.Boutet, P.Fromme, T.A.White, A.Barty, J.C.Spence, U.Weierstall, W.Liu, V.Cherezov.

ABSTRACT

Serial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs) enables high-resolution protein structure determination using micrometre-sized crystals at room temperature with minimal effects from radiation damage. SFX requires a steady supply of microcrystals intersecting the XFEL beam at random orientations. An LCP-SFX method has recently been introduced in which microcrystals of membrane proteins are grown and delivered for SFX data collection inside a gel-like membrane-mimetic matrix, known as lipidic cubic phase (LCP), using a special LCP microextrusion injector. Here, it is demonstrated that LCP can also be used as a suitable carrier medium for microcrystals of soluble proteins, enabling a dramatic reduction in the amount of crystallized protein required for data collection compared with crystals delivered by liquid injectors. High-quality LCP-SFX data sets were collected for two soluble proteins, lysozyme and phycocyanin, using less than 0.1 mg of each protein.