PDBsum entry 4ziy

Ligase

PDB id: 4ziy

Contents

Protein chain

446 a.a.

Ligands

ANP

EDO ×3

Metals

_MG

Waters ×315

PDB id:

4ziy

Name:

Ligase

Title:

Structure of udp-n-acetylmuramoylalanyl-d-glutamyl-2,6- diaminopimelate--d-alanyl-d-alanyl ligase from acinetobacter baumannii

Structure:

Udp-n-acetylmuramoyl-tripeptide--d-alanyl-d-alanine ligase. Chain: a. Engineered: yes

Source:

Acinetobacter baumannii ab5075. Organism_taxid: 1116234. Strain: ab5075-uw. Gene: murf, a591_a0258. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

1.85Å R-factor: 0.175 R-free: 0.202

Authors:

Seattle Structural Genomics Center For Infectious Disease (Ssgcid)

Key ref:

Seattle structural genomics center for infectious dis(ssgcid) et al. Structure of udp-N-Acetylmuramoylalanyl-D-Glutamyl-2, 6-Diaminopimelate--D-Alanyl-D-Alanyl ligase from acinetobacter baumannii. To be published, .

Date:

28-Apr-15 Release date: 20-May-15

Protein chain

A0A0D5YEC3  (A0A0D5YEC3_ACIBA) -  UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase from Acinetobacter baumannii

Seq: 466 a.a.

Struc: 446 a.a.

Enzyme reactions

• Enzyme class: E.C.6.3.2.10 - UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase.
• Pathway: Peptidoglycan Biosynthesis (Part 1)
• Reaction: UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-alanyl-D-alanine + ATP = UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma- D-glutamyl-L-lysyl-D-alanyl-D-alanine + ADP + phosphate + H⁺

UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-alanyl-D-alanine + ATP = UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma- D-glutamyl-L-lysyl-D-alanyl-D-alanine (Bound ligand (Het Group name = ANP) matches with 81.25% similarity) + ADP + phosphate + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site