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PDBsum entry 4zix
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PDB id:
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Hydrolase
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Title:
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Structure of hewl using serial femtosecond crystallography of soluble proteins in lipidic cubic phase
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Structure:
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LysozymE C. Chain: a. Synonym: 1,4-beta-n-acetylmuramidasE C,allergen gal d iv. Engineered: yes
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Source:
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Gallus gallus. Chicken. Organism_taxid: 9031. Gene: lyz. Expressed in: escherichia coli. Expression_system_taxid: 562
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Resolution:
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1.89Å
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R-factor:
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0.167
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R-free:
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0.191
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Authors:
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R.Fromme,A.Ishchenko,M.Metz,S.Roy-Chowdhury,S.Basu,S.Boutet,P.Fromme, T.A.White,A.Barty,J.C.H.Spence,U.Weierstall,W.Liu,V.Cherezov
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Key ref:
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R.Fromme
et al.
(2015).
Serial femtosecond crystallography of soluble proteins in lipidic cubic phase.
Iucrj,
2,
545-551.
PubMed id:
DOI:
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Date:
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28-Apr-15
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Release date:
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12-Aug-15
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PROCHECK
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Headers
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References
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P00698
(LYSC_CHICK) -
Lysozyme C from Gallus gallus
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Seq:
Struc:
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147 a.a.
129 a.a.
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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Enzyme class:
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E.C.3.2.1.17
- lysozyme.
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Reaction:
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Hydrolysis of the 1,4-beta-linkages between N-acetyl-D-glucosamine and N-acetylmuramic acid in peptidoglycan heteropolymers of the prokaryotes cell walls.
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DOI no:
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Iucrj
2:545-551
(2015)
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PubMed id:
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Serial femtosecond crystallography of soluble proteins in lipidic cubic phase.
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R.Fromme,
A.Ishchenko,
M.Metz,
S.R.Chowdhury,
S.Basu,
S.Boutet,
P.Fromme,
T.A.White,
A.Barty,
J.C.Spence,
U.Weierstall,
W.Liu,
V.Cherezov.
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ABSTRACT
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Serial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs)
enables high-resolution protein structure determination using micrometre-sized
crystals at room temperature with minimal effects from radiation damage. SFX
requires a steady supply of microcrystals intersecting the XFEL beam at random
orientations. An LCP-SFX method has recently been introduced in which
microcrystals of membrane proteins are grown and delivered for SFX data
collection inside a gel-like membrane-mimetic matrix, known as lipidic cubic
phase (LCP), using a special LCP microextrusion injector. Here, it is
demonstrated that LCP can also be used as a suitable carrier medium for
microcrystals of soluble proteins, enabling a dramatic reduction in the amount
of crystallized protein required for data collection compared with crystals
delivered by liquid injectors. High-quality LCP-SFX data sets were collected for
two soluble proteins, lysozyme and phycocyanin, using less than 0.1 mg of each
protein.
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