PDBsum entry 4zhy

Transcription/signaling protein

PDB id: 4zhy

Contents

Protein chains

150 a.a.

112 a.a.

Ligands

SO4 ×2

FMT ×4

Waters ×160

PDB id:

4zhy

Name:

Transcription/signaling protein

Title:

Crystal structure of a bacterial signalling complex

Structure:

Yfir. Chain: a. Engineered: yes. Mutation: yes. Yfib. Chain: b. Engineered: yes

Source:

Pseudomonas aeruginosa pao1. Organism_taxid: 208964. Strain: pao1. Gene: yfir. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: yfib. Expression_system_taxid: 562

Resolution:

1.97Å R-factor: 0.183 R-free: 0.219

Authors:

S.Li,T.Li,Y.Wang,M.Bartlam

Key ref:

S.Li et al. (2015). Structural insights into YfiR sequestering by YfiB in Pseudomonas aeruginosa PAO1. Sci Rep, 5, 16915. PubMed id: 26593397 DOI: 10.1038/srep16915

Date:

27-Apr-15 Release date: 27-Apr-16

Protein chain

Q9I4L4  (Q9I4L4_PSEAE) -  Negative regulator YfiR from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

Seq: 190 a.a.

Struc: 150 a.a.*

Protein chain

Q9I4L6  (Q9I4L6_PSEAE) -  Outer-membrane lipoprotein YfiB from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

Seq: 168 a.a.

Struc: 112 a.a.*

* PDB and UniProt seqs differ at 4 residue positions (black crosses)

DOI no: 10.1038/srep16915

Sci Rep 5:16915 (2015)

PubMed id: 26593397

Structural insights into YfiR sequestering by YfiB in Pseudomonas aeruginosa PAO1.

S.Li, T.Li, Y.Xu, Q.Zhang, W.Zhang, S.Che, R.Liu, Y.Wang, M.Bartlam.

ABSTRACT

YfiBNR is a tripartite signalling system in Pseudomonas aeruginosa that modulates intracellular c-di-GMP levels in response to signals received in the periplasm. YfiB is an outer membrane lipoprotein and presumed sensor protein that sequesters the repressor protein YfiR. To provide insights into YfiBNR function, we have determined three-dimensional crystal structures of YfiB and YfiR from P. aeruginosa PAO1 alone and as a 1:1 complex. A YfiB(27-168) construct is predominantly dimeric, whereas a YfiB(59-168) is monomeric, indicating that YfiB can dimerize via its N-terminal region. YfiR forms a stable complex with YfiB(59-168), while the YfiR binding interface is obstructed by the N-terminal region in YfiB(27-168). The YfiB-YfiR complex reveals a conserved interaction surface on YfiR that overlaps with residues predicted to interact with the periplasmic PAS domain of YfiN. Comparison of native and YfiR-bound structures of YfiB suggests unwinding of the N-terminal linker region for attachment to the outer membrane. A model is thus proposed for YfiR sequestration at the outer membrane by YfiB. Our work provides the first detailed insights into the interaction between YfiB and YfiR at the molecular level and is a valuable starting point for further functional and mechanistic studies of the YfiBNR signalling system.