PDBsum entry 4zgi

Signaling protein

PDB id: 4zgi

Contents

Protein chain

140 a.a.

Waters ×8

PDB id:

4zgi

Name:

Signaling protein

Title:

Structure of truncated human tifa

Structure:

Traf-interacting protein with fha domain-containing protein a. Chain: a. Fragment: unp residues 10-149. Synonym: putative mapk-activating protein pm14,putative nf-kappa-b- activating protein 20,traf2-binding protein. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: tifa, t2bp. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.70Å R-factor: 0.230 R-free: 0.264

Authors:

J.H.Weng,T.Y.W.Wei,Y.C.Hsieh,C.C.F.Huang,P.Y.G.Wu,E.S.W.Chen, K.F.Huang,C.J.Chen,M.D.Tsai

Key ref:

J.H.Weng et al. (2015). Uncovering the Mechanism of Forkhead-Associated Domain-Mediated TIFA Oligomerization That Plays a Central Role in Immune Responses. Biochemistry, 54, 6219-6229. PubMed id: 26389808 DOI: 10.1021/acs.biochem.5b00500

Date:

23-Apr-15 Release date: 14-Oct-15

Protein chain

Q96CG3  (TIFA_HUMAN) -  TRAF-interacting protein with FHA domain-containing protein A from Homo sapiens

Seq: 184 a.a.

Struc: 140 a.a.

DOI no: 10.1021/acs.biochem.5b00500

Biochemistry 54:6219-6229 (2015)

PubMed id: 26389808

Uncovering the Mechanism of Forkhead-Associated Domain-Mediated TIFA Oligomerization That Plays a Central Role in Immune Responses.

J.H.Weng, Y.C.Hsieh, C.C.Huang, T.Y.Wei, L.H.Lim, Y.H.Chen, M.R.Ho, I.Wang, K.F.Huang, C.J.Chen, M.D.Tsai.

ABSTRACT

Forkhead-associated (FHA) domain is the only signaling domain that recognizes phosphothreonine (pThr) specifically. TRAF-interacting protein with an FHA domain (TIFA) was shown to be involved in immune responses by binding with TRAF2 and TRAF6. We recently reported that TIFA is a dimer in solution and that, upon stimulation by TNF-α, TIFA is phosphorylated at Thr9, which triggers TIFA oligomerization via pThr9-FHA domain binding and activates nuclear factor κB (NF-κB). However, the structural mechanism for the functionally important TIFA oligomerization remains to be established. While FHA domain-pThr binding is known to mediate protein dimerization, its role in oligomerization has not been demonstrated at the structural level. Here we report the crystal structures of TIFA (residues 1-150, with the unstructured C-terminal tail truncated) and its complex with the N-terminal pThr9 peptide (residues 1-15), which show unique features in the FHA structure (intrinsic dimer and extra β-strand) and in its interaction with the pThr peptide (with residues preceding rather than following pThr). These structural features support previous and additional functional analyses. Furthermore, the structure of the complex suggests that the pThr9-FHA domain interaction can occur only between different sets of dimers rather than between the two protomers within a dimer, providing the structural mechanism for TIFA oligomerization. Our results uncover the mechanism of FHA domain-mediated oligomerization in a key step of immune responses and expand the paradigm of FHA domain structure and function.