PDBsum entry 4zft

Hydrolase

PDB id: 4zft

Contents

Protein chains

185 a.a.

78 a.a.

Ligands

EDO ×5

Metals

_ZN ×4

Waters ×79

PDB id:

4zft

Name:

Hydrolase

Title:

Catalytic domain of sst2 f403w mutant bound to ubiquitin

Structure:

Amsh-like protease sst2. Chain: a, c. Fragment: unp residues 245-435. Synonym: suppressor of ste12 deletion protein 2. Engineered: yes. Mutation: yes. Polyubiquitin-b. Chain: b, d. Fragment: unp residues 77-152.

Source:

Schizosaccharomyces pombe. Fission yeast. Organism_taxid: 284812. Strain: 972 / atcc 24843. Gene: sst2, spac19b12.10. Expressed in: escherichia coli. Expression_system_taxid: 562. Homo sapiens. Human.

Resolution:

2.30Å R-factor: 0.194 R-free: 0.235

Authors:

A.N.Bueno

Key ref:

A.N.Bueno Structure of the catalytic domain of sst2 mutant f403 to ubiquitin at 2.3 angstroms. To be published, .

Date:

21-Apr-15 Release date: 14-Oct-15

Protein chains

Q9P371  (SST2_SCHPO) -  AMSH-like protease sst2 from Schizosaccharomyces pombe (strain 972 / ATCC 24843)

Seq: 435 a.a.

Struc: 185 a.a.*

Protein chains

P0CG47  (UBB_HUMAN) -  Polyubiquitin-B from Homo sapiens

Seq: 229 a.a.

Struc: 78 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class 2: Chains A, C: E.C.3.4.19.- - ?????
• Enzyme class 3: Chains B, D: E.C.?

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.